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Human ICAM-1/CD54(intercellular adhesion molecule 1) ELISA Kit

Citations(10)Uniprot : P05362

Cat.No.:E-EL-H6114
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Human ICAM-1/CD54 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human ICAM-1/CD54 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ICAM-1/CD54. You can calculate the concentration of Human ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ICAM-1/CD54 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ICAM-1/CD54 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.00	2.42	8.44	0.84	2.29	8.45
Standard deviation	0.06	0.12	0.34	0.05	0.10	0.34
CV (%)	6.00	4.96	4.03	5.95	4.37	4.02

Recovery

The recovery of Human ICAM-1/CD54 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-98	94
EDTA plasma (n=8)	88-98	95
Cell culture media (n=8)	86-101	94

Linearity

Samples were spiked with high concentrations of Human ICAM-1/CD54 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05362
Synonyms	CD54
Research Area	Cancer, Cardiovascular, Immunology, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Nutrients (2021) IF: 5.719

Rapid and Effective Vitamin D Supplementation May Present Better Clinical Outcomes in COVID-19 (SARS-CoV-2) Patients by Altering Serum INOS1, IL1B, IFNg, Cathelicidin-LL37, and ICAM1

DOI: 10.3390/nu13114047
PMID: 34836309	Sample: serum

JOURNAL OF PROTEOME RESEARCH (2021) IF: 4.466

Proteomic Profiling of Leukocytes Reveals Dysregulation of Adhesion and Integrin Proteins in Chronic Kidney Disease-Related Atherosclerosis

DOI: 10.1021/acs.jproteome.0c00883
PMID: 33939431	Sample: Cell culture supernatant,Plasma

Oxidative Medicine and Cellular Longevity (2019) IF: 4.868

Antitumoral and Immunomodulatory Effect of Mahonia aquifolium Extracts

DOI: 10.1155/2019/6439021
	Sample: A2780 cell,A375 cell,BJ cell,DLD-1 cell

DYES AND PIGMENTS (2022) IF: 4.889

New methylene blue analogues with N-piperidinyl-carbinol units: Synthesis, optical properties and in vitro internalization in human ovarian cancer cells

DOI: 10.1016/j.dyepig.2022.110460
	Sample: A2780 cell,OVCAR-3 cell,BJ cell

Oncology Letters (2022) IF: 3.111

A role for serum cytokines and cell adhesion molecules in the non‑invasive diagnosis of colorectal cancer

DOI: 10.3892/ol.2022.13443
	Sample: serum

BRITISH JOURNAL OF NUTRITION (2022) IF: 3.718

Brazil and cashew nuts intake improve body composition and endothelial health in women at cardiometabolic risk (Brazilian Nuts Study): a randomized controlled trial

DOI: 10.1017/S000711452100475X
PMID: 35193718	Sample: serum

JOURNAL OF CLINICAL LABORATORY ANALYSIS (2022) IF: 2.352

Biomarkers of connective tissue disease-associated interstitial lung disease in bronchoalveolar lavage fluid: A label-free mass spectrometry-based relative quantification study

DOI: 10.1002/jcla.24367
PMID: 35334492	Sample: Bronchoalveolar lavage fluid (BALF)

Experimental and Therapeutic Medicine (2023) IF: 2.751

NEDD4 attenuates oxidized low‑density lipoprotein‑induced inflammation and dysfunction in vascular endothelial cells via regulating APEX1 expression

DOI: 10.3892/etm.2023.11787
	Sample: U937 monocytes,human umbilical vein endothelial ce

Journal of Obsessive-Compulsive and Related Disorders (2020) IF: 1.418

Decreased plasma intercellular adhesion molecule-3 levels are associated with obsessive compulsive disorder in medication-free children

DOI: 10.1016/j.jocrd.2020.100581
	Sample: plasma

Cellular Reprogramming (2021) IF: 1.987

Comparison of the Cytokine Profile in Mesenchymal Stem Cells from Human Adipose, Umbilical Cord, and Placental Tissues

DOI: 10.1089/cell.2021.0043
PMID: 34677101	Sample: mesenchymal stem cells (MSCs)

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