Current Position:Home>>Kits>>Metabolism Assays>> Lactase Activity Assay Kit

Lactase Activity Assay Kit

Cat.No.:E-BC-K131-M
Detection instrument: Microplate reader(495 nm-510 nm,optimum wavelength: 505 nm)

Detection method: Colorimetric method

To Purchase E-BC-K131-M

Size:

96T

Price: $200

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Lactase decomposes lactose to produce glucose. Under the action of enzyme, glucose produces hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to produce colored substances. Lactase activity can be calculated by measuring the OD value at 505 nm.

Performance characteristics

Sample type	Animal tissue
Sensitivity	3.94 U/mL
Detection range	12.5-2000 U/mL.
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	60 min
Precision	Average inter-assay CV: 8.500%Average intra-assay CV: 4.500%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

The activity of lactase in pig jejunum was significantly changed by different daily diet.

H M J Hees et al investigate the supplementation of oat hulls in piglets colonic microbiota development. Activity of lactase in pig jejunum was determined using lactase activity assay kit (E-BC-K131-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (12.5-2000 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Rat lleal tissue homogenate	1
10% Rat jejunum tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Cellular and Molecular Gastroenterology and Hepatology (2021) IF: 9.225

Aberrant Epithelial Differentiation Contributes to Pathogenesis in a Murine Model of Congenital Tufting Enteropathy

DOI: 10.1016/j.jcmgh.2021.06.015
PMID: 34198013	Sample: duodenal

Animal Nutrition (2022) IF: 5.285

Supplementing oat hulls to the diet of suckling piglets altered their intestinal tract and colonic microbiota development

DOI: 10.1016/j.aninu.2022.10.002
	Sample: jejunum

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

Cat. No	E-BC-K131-M
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha