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Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE Anti-Rat CD45RA Antibody[OX-33]

PE Anti-Rat CD45RA Antibody[OX-33]

  • Cat.No.:E-AB-F1306D

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse
Applications:

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  • 100Tests×2
Price: $80
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Antigen Details

Alternate Names Leukocyte common antigen, high molecular weight form
Gene ID 24699
References
1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168.

Product Details

Form Liquid
Clone No. OX-33
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PE Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792D]
Reactivity Rat
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with a molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Rat CD45RA Antibody[OX-33] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Rat CD45RA Antibody[OX-33] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Rat CD45RA Antibody[OX-33] 50Tests, 100Tests, 100Tests×2 Check More

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