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Plant Nitrate Nitrogen Colorimetric Assay Kit

Cat.No.:E-BC-K858-M
Detection instrument: Microplate reader (405-415 nm, optimum wavelength: 410 nm)

Detection method: Colorimetric method

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48T

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Detection principle

Nitrate nitrogen can react with salicylic acid to produce nitrosalicylic acid in a strongly acidic environment. Nitrosalicylic acid is yellow under strong alkaline conditions, and the depth of color is proportional to the content at a wavelength of 410 nm.

Performance characteristics

Sample type	plant tissue;soil
Sensitivity	1.09 μg/mL
Detection range	1.09-200 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 3.2-5.1%Average intra-assay CV: 1.5-4.3%
Other instruments required	Incubator, Vortex mixer, Centrifuge
Other reagents required	Potassium nitrate, Concentrated sulfuric acid
Storage	2-8℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K177-M	Proline (Pro) Colorimetric Assay Kit
E-BC-K860-M	Proline Dehydrogenase (ProDH) Activity Assay Kit

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Collection and treatment of common samples for metabolism assay detection

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