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Cat.No.:PKSV030236
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Synonyms | coronavirus s1 Protein;MERS-CoV;coronavirus s2 Protein;MERS-CoV;coronavirus spike Protein;MERS-CoV;cov spike Protein;MERS-CoV;ncov RBD Protein;MERS-CoV;ncov s1 Protein;MERS-CoV;ncov s2 Protein;MERS-CoV;ncov spike Protein;MERS-CoV;RBD Protein;MERS-CoV;S Protein;MERS-CoV;s1 Protein;MERS-CoV;Spike RBD Protein;MERS-CoV |
Species | MERS-CoV |
Expression_host | Baculovirus-Insect Cells |
Sequence | Met1-Trp1297 |
Accession | AFS88936.1 |
Mol_Mass | 142.5 kDa |
Tag | C-His |
Bio_Activity | Measured by its binding ability in a functional ELISA. 1. Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat:PKSH033811), The EC50 of can biotinylated DPP4 (Cat:PKSH033811) is 0.02-0.04 ug/mL. 2. Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat:PKSH030456), The EC50 of can biotinylated Fc-DPP4 (Cat:PKSH030456) is 0.01-0.02 ug/mL. |
Purity | > 85 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |