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Cat.No.:E-BC-K298-M
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Synonyms | TBARS |
Sample type | Serum,plasma,animal tissue |
Sensitivity | 0.85 μmol/L |
Detection range | 2.6-100 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 80 min |
Precision | Average inter-assay CV: 7.100%Average intra-assay CV: 4.300% |
Other instruments required | Vortex mixer, Micropipettor, Water bath |
Other reagents required | Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
High dose EGCG increased the concentration of TBARS in rat liver. (*P<0.05)
KW Parn et al investigate the anti-hypertension effect of epigallocatechin gallate. Thiobarbituric acid reactants (TBARS) level in rat liver was determined using TBARS colorimetric assay kit (E-BC-K298-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.6-100 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Rat serum |
1 |
10% Mouse brain tissue homogenization |
1 |
Human serum |
1 |
Rat serum |
1 |
10% Rat liver tissue homogenization |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
PMID: 33512269 |
Sample: Plasma |
DOI: 10.1002/fsn3.2875 |
|
Sample: Raw264.7 Cell |
PMID: 32249612 |
Sample: Urine |
Sample: Kle Cell |
DOI: 10.3390/pr10071314 |
|
Sample: Stomach |
PMID: 33310035 |
Sample: Tissue |
PMID: 31972360 |
Sample: Tissue Homogenate |
DOI: 10.3390/ijms23074015 |
|
PMID: 35409375 |
Sample: Serum |
PMID: 32179202 |
Sample: Tissue Homogenate |
Sample: Artificial Serum |
DOI: 10.3390/nu14214605 |
|
Sample: Liver |
M*****iSubmitted [ Aug 02 2022 ]
Asked: Do I need to prepare the acetic acid by myself?
Reply
adminSubmitted [ Aug 02 2022 ]
Answered: Yes, you do.