WST-8 is a compound similar to MTT, which can be reduced to orange formazan by some dehydrogenase in mitochondria in the presence of electron coupling reagent. The amount of formazan produced is directly proportional to the number of living cells. By measuring the absorbance at 450 nm, the amount of living cells can be calculated indirectly.

General Information

Application	Viability/Proliferation/Cytotoxicity
Detection method	Colorimetric Method
Sample type	Cell samples
Detection instrument	Microplate reader
Storage	2~8°C or -20°C , shading light
Shipping	Ice bag
Expiration date	24 months

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Citations

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DOI: 10.3390/ijms232416105

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DOI: 10.1021/acschembio.2c00131
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Verbascoside potentiates the effect of tyrosine kinase inhibitors on the induction of apoptosis and oxidative stress via the Abl-mediated MAPK signalling pathway in chronic myeloid leukaemia

DOI: 10.3892/etm.2022.11441
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DOI: 10.3892/etm.2021.10407

Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2022) IF: 2.464

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DOI: 10.1186/s41065-022-00241-0
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DOI: 10.1186/s40662-022-00291-5
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DOI: 10.1016/j.bbrc.2022.08.040
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Guizhi-Shaoyao-Zhimu decoction attenuates bone erosion in rats that have collagen-induced arthritis via modulating NF‐κB signalling to suppress osteoclastogenesis

DOI: 10.1080/13880209.2021.1876100

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DOI: 10.2147/CMAR.S359545
PMID: 36117731

PLoS One (2023) IF: 3.752

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DOI: 10.1371/journal.pone.0281170

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DOI: 10.1093/jpp/rgac035
PMID: 35640566

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DOI: 10.1016/j.phytochem.2022.113577

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The PTBP1‑NCOA4 axis promotes ferroptosis in liver cancer cells

DOI: 10.3892/or.2023.8482

Human Cell (2022) IF: 4.174

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DOI: 10.1007/s13577-022-00709-1
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MOLECULES (2021) IF: 4.412

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DOI: 10.3390/molecules26216366
PMID: 34770773

MOLECULES (2022) IF: 4.412

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DOI: 10.3390/molecules27051706

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556

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DOI: 10.3390/ijms21196991
PMID: 32977456	Sample: Cell Sample

BMC CANCER (2022) IF: 4.638

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DOI: 10.1186/s12885-022-09963-w
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MOLECULES (2022) IF: 4.927

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Scientific Reports (2023) IF: 4.996

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DOI: 10.1038/s41598-023-29546-4

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DOI: 10.1016/j.ceramint.2022.10.296

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DOI: 10.3389/fphar.2021.670054
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DOI: 10.1016/j.phymed.2022.154329
PMID: 35843187

Materials Today Bio (2021) IF: 7.348

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DOI: 10.1016/j.mtbio.2021.100189

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DOI: 10.1016/j.biopha.2022.113884
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DOI: 10.3389/fimmu.2022.1100417

Frontiers in Immunology (2022) IF: 8.786

AQP2 Promotes Astrocyte Activation by Modulating the TLR4/NFκB-p65 Pathway Following Intracerebral Hemorrhage.

DOI: 10.3389/fimmu.2022.847360

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DOI: 10.1016/j.mtbio.2022.100326
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JOURNAL OF PINEAL RESEARCH (2022) IF: 12.081

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DOI: 10.1111/jpi.12819
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Polystyrene nanoparticle exposure supports ROS-NLRP3 axis-dependent DNA-NET to promote liver inflammation

DOI: 10.1016/j.jhazmat.2022.129502
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Enhanced Cell Counting Kit 8 (WST-8/CCK8)

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Detection principle