MTT is 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide which can be reduced by some dehydrogenases in mitochondria to form a crystalline dark purple product formazan. Formazan can be completely dissolved by DMSO and its absorbance is near 570 nm wavelength. The more and faster the cells proliferate, the darker the color is, and the more cytotoxic, the lighter the color is. For the same cells, there is a linear relationship between the depth darkness of color and the number of cells.

General Information

Application	Viability/Proliferation/Cytotoxicity
Detection method	Colorimetric Method
Sample type	Cell samples
Detection instrument	Microplate reader
Other reagents required	DMSO
Storage	2~8°C, shading light
Shipping	Ice bag
Expiration date	6 months

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Citations

SURFACE ENGINEERING (2020) IF: 2.433

Ag-containing antibacterial self-healing micro-arc oxidation coatings on Mg–Zn–Sr alloys

DOI: 10.1080/02670844.2020.1800346
	Sample: Cell Culture Supernatant

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL (2021) IF: 2.9

Catechin Metabolites along with Curcumin Inhibit Proliferation and Induce Apoptosis in Cervical Cancer Cells by Regulating VEGF Expression In-Vitro

DOI: 10.1080/01635581.2021.1936082
PMID: 34121550

Photodiagnosis and Photodynamic Therapy (2021) IF: 3.631

Influence of antibacterial effects of tetracycline, laser, and photodynamic therapy on cell viability, cell damage, and virulence of Porphyromonas gingivalis.

DOI: 10.1016/j.pdpdt.2021.102617

BMC Complementary and Alternative Medicine (2022) IF: 3.659

Evaluation of the antimalarial activity and toxicity of Mahanil-Tang-Thong formulation and its plant ingredients

DOI: 10.1186/s12906-022-03531-2
PMID: 35219319

MOLECULES (2022) IF: 4.927

Oncolytic Newcastle Disease Virus Co-Delivered with Modified PLGA Nanoparticles Encapsulating Temozolomide against Glioblastoma Cells: Developing an Effective Treatment Strategy

DOI: 10.3390/molecules27185757
PMID: 36144488

MOLECULES (2023) IF: 4.927

Enhanced Intracellular Photosensitizer Uptake and Retention by Targeting Viral Oncoproteins in Human Papillomavirus Infected Cancer Cells and Cancer Stem Cells

DOI: 10.3390/molecules28020647

FASEB JOURNAL (2020) IF: 4.966

CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

DOI: 10.1096/fj.201902910RRR
	Sample: Cell

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