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Fructose Fluorometric Assay Kit

  • Cat.No.:E-BC-F080

  • Detection instrument: Fluorescence microplate reader (Ex/Em=530 nm/590 nm)

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  • 96T
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Detection principle

Fructose is the most common hexketoose, which is often present in large amounts as a free monosaccharide in fruits and honey. A large number of epidemiological data and experimental studies have shown that excessive fructose intake may be an important factor in the increased incidence of metabolic diseases. Fructose can produce a specific product under the action of enzymes, which reacts with the chromogenic agent to produce a fluorescent substance (Ex/Em: 530 nm/590 nm).

Performance characteristics

Sample type serum;plasma;tissue
Sensitivity 0.72 μmol/L
Detection range 0.72-500 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 150 min
Precision Average inter-assay CV: 3.2-7.3%Average intra-assay CV: 1.7-3.1%
Other instruments required Incubator (37℃), Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage -20℃
Valid period 12 months

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