Prokaryotic protein expression system is the most common and
economical protein expression method today, and the E. coli expression system is one of the most important
and widely used prokaryotic protein expression systems. Elabscience's E. coli protein expression system
service starts with our proprietary codon optimization and protein
analysis (S-CP-P001), then gene will
be synthesised and subcloned (S-CP-P002), there are all kinds of pET series vectors and integrate GST or MBP tags to select
according to customer needs, and there is tag removal service (S-CP-P005), during the transformation and small test, multiple
expression strains such as BL21 (DE3), T7 Express, Rosseta gami (DE3) plys, Shuffle T7B, BL21 Codon Plus
(DE3) are provided (S-CP-P003). In
addition, we provide endotoxin removal service (S-CP-P008) to meet your special requirements. You can order each service or any
combination of these services according to the project plan.
Submit your project information and requirements and place an order.
Our experienced experts will help you with your research and promise to provide you with quality services at a
competitive price.
Advantages
Competitive price: Starting from $1,200.
Short period: As short as 5 weeks.
Guaranteed
Package: 1~5 mg of purified
proteins with a competitive price of $1,980.
High-purity protein
protein production: A variety of purification methods combined with protein purification system can customize purity
≥95%.
One-stop service
platform: Elabscience® can take your project directly from gene synthesis to downstream activities including recombinant protein
expression, antibody production, and assay development.
E.coli Expression and Purification Service (E.coli System) Details
|
Cat. No.
|
Service Name
|
Timeline
|
Delivery
|
Quoted price
|
|
S-CP-P001
|
Analysis of gene and target protein
|
1-2 days
|
|
Free
|
|
S-CP-P002
|
Gene synthesis and preparation of
expression vector
|
2-3 weeks
|
-
Expression vector containing the
target gene
-
Target sequencing report
-
Escherichia coli containing expression vector
plasmid
-
Related analysis reports
|
$0.4/bp
|
|
S-CP-P003
|
Transform and optimized expression condition
|
1-2 weeks
|
-
The optimum inducing conditions (temperature,
time, concentration of IPTG)
-
Analysis of protein solubility
|
$460
|
|
S-CP-P004
|
Large scale expression in 1L and tag affinity
purification
|
2-3 weeks
|
-
1L bacterial expression, purity≥80% of target
protein fused with His tag
-
Related analysis reports
|
$460（The price of each additional 1L increases
$400）
|
|
S-CP-P005
|
Tag removal and subsequent purification
|
1-2 weeks
|
-
1L
bacterial expression, purity≥85%, without tag target protein
-
Enzyme digestion test and purification
report
|
$460
|
|
S-CP-P006
|
The secondary purification
|
1 week
|
-
Purity≥90%, total 1-5mg target protein
-
Related analysis reports
|
$400
|
|
S-CP-P007
|
Final purification
|
1 week
|
-
Purity≥95%, total 1-5mg target protein
-
Related analysis reports
|
$500
|
|
S-CP-P008
|
Endotoxin removal
|
1 week
|
-
1-5mg target protein, endotoxin < 1Eu /μg
|
$500
|
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