Proteinase K Lyophilized Powder

Introduction

Proteinase K is a kind of high activity Proteinase of subtilisin, which is used to degrade proteins in biological samples. It can be used to digest various proteins, and it can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on.

Enzyme activity, > 30 U/mg. At 37°C, the amount of Proteinase K that can produce amino acids or polypeptides equivalent to 1 micromol of tyrosine Folin positive in one minute with hemoglobin as the substrate is defined as a unit of Proteinase K activity.

The effective pH range of Proteinase K is pH4.0~12.5, and the optimal pH range is pH7.5~8.0.

The optimum reaction temperature of Proteinase K is 65°C, but at 65°C or higher, Proteinase K can also degrade rapidly. It is suggested that the optimum reaction temperature is 50~55°C.

Components

Specification parameters

Applications

·  DNA, RNA, and phage purification

·  Protein digestion

·  Chromosomal DNA preparation for in situ hybridization, fingerprinting, colony or plaque hybridization, and pulse-field electrophoresis

Usage

Reconstitution. Dissolve the powder with ddH₂O. It is recommended to keep the solution at 2~8°C.

Use it as soon as possible after opening and please avoid pollution.

Storage

Store at RT or lower temperature.

FAQs for Proteinase K

1.    Can I use the Proteinase K for molecular biology applications?

The Proteinase K is free of exonuclease and endonuclease activity, RNase activity, and contains no detectable DNA (both bacterial and eukaryotic) contamination. Therefore, it is ideally suited for molecular biology and genomic research applications.

2.    What buffer should I use to dilute the Proteinase K Lyophilized Powder?

Dissolve the powder with 50% glycerin or ddH₂O to 1X reaction buffer(Follow the manual). It is recommended to keep the solution at 2~8°C for 3 months.

To create a stock that permits longer-term storage, Please dissolve the powder  at 10~20 mg/mL and store at -20°C.

3.    What is the optimal reaction condition for the Proteinase K?

The Proteinase K is compatible with a wide range and the optimal pH is 7.5~8.0, optimal temperature is 50~55°C and the optimal concentration is 50~100 μg/mL.

4.    Is there any Proteinase K Reagent product?

Yes. We do have the Proteinase K Reagent (Cat# E-IR-R109), and the concentration is 10 mg/mL.

5.    What are the different between the proteinase K Reagent and Proteinase K Lyophilized Powder?

They are the same product in different character. Powder is more stable for transport and storage. And the Reagent is a concentrate proteinase K at 10 mg/mL.