Detection of T/B/NK (4-color) in human peripheral blood - Elabscience

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Detection of T/B/NK (4-color) in human peripheral blood


Purpose

Sample

Antibody Collocation

Adjust the voltage

1

Blank

Adjust compensation

2

CD45-PerCP

3

CD3-FITC

4

CD16-PE/ CD56-PE

5

CD19-APC

PE-FMO in combination with Isotype Control for auxiliary gating

6

CD45-PerCP, CD3-FITC, CD19-APC; Mouse IgG1, κ Isotype Control-PE

Full panel

7

CD45-PerCP, CD3-FITC, CD16-PE, CD56-PE, CD19-APC

Marker

Fluorochrome

Clone No.

Cat. No.

CD45

PerCP

HI30

E-AB-F1137F

CD3

FITC

OKT3

E-AB-F1001C

CD16

PE

3G8

E-AB-F1236D

CD56

PE

5.1H11

E-AB-F1239D

CD19

APC

CB19

E-AB-F1004E

Mouse IgG1, κ Isotype Control

PE

MOPC-21

E-AB-F09792D



Tips:

1.  This panel has obvious cell populations, and compensation regulation can be performed without single positive tube. But for beginners of Flow Cytometry, it is recommended to set up a single positive tube to adjust compensation.

2.  The detection standard for NK cells is CD3-CD16+CD56+.

3.  In this panel, it is recommended to set Isotype Control for CD16 and CD56, while other indicators can be omitted due to obvious populations.

4.  It is recommended to stain human peripheral blood samples with CD45, which is beneficial for the lymphocyte phylum gating through CD45 and SSC. It is recommended to use the single positive tube of CD45 to start the machine at low speed and set the threshold.





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