Detection of T/B/NK (3-color) in Rat Spleen - Elabscience

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Cell Types

T/B/NK Cell Population Detection

Detection of T/B/NK (4-color) in human peripheral blood
Detection of T/B/NK (4-color) in C57BL/6 mouse peripheral blood
Detection of TB cells (4-color) in mouse lymph node
Detection of T/B/NK (5-color) in Rat Spleen
Detection of T/B/NK (3-color) in Rat Spleen
CD4+/CD8+T Cell Population Detection

Detection of T cells (4-color) in Human Peripheral Blood
Detection of T Cells (2-color) in Mouse Thymus
Detection of Lymphocyte (4 colors) in Mouse Tumor
Treg Cell Population Detection

Detection of Treg (3-color) in Mouse Spleen
Detection of Treg (6-color) in Human Peripheral Blood
Th1/Th2/ Th17 Cell Population Detection

Detection of Th1/Th2 (4-color) in Human Peripheral Blood PBMC
Detection of Th17 (3-color) in Human Peripheral Blood PBMC

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Detection of T/B/NK (3-color) in Rat Spleen

Panel Design

Purpose	Sample	Antibody Collocation
Adjust the voltage	1	Blank
Adjust compensation	2	CD3-FITC
	3	CD45RA-PE
	4	CD161-APC
APC-FMO in combination with Isotype Control for auxiliary gating	5	CD3-FITC, CD45RA-PE; Mouse IgG1, κ Isotype Control-APC
PE-FMO in combination with Isotype Control for auxiliary gating	6	CD3-FITC, CD161-APC; Mouse IgG1, κ Isotype Control-PE
Full panel	7	CD3-FITC, CD45RA-PE, CD161-APC

Information of Flow Cytometry Antibodies

Marker	Fluorochrome	Clone No.	Cat. No.
CD3	FITC	G4.18	E-AB-F1228C
CD45RA	PE	OX-33	E-AB-F1306D
CD161	APC	3.2.3	E-AB-F1307E
Mouse IgG1, κ Isotype Control	PE	MOPC-21	E-AB-F09792D
Mouse IgG1, κ Isotype Control	APC	MOPC-21	E-AB-F09792E

Flow Cytometry Result

Tips:

1. The phenotype of rat B cells is CD3^-CD45RA⁺, and the phenotype of NK cells is CD3^-CD161⁺.

2. Isotype Control for CD45RA and CD161 is suggested for auxiliary gating.

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