Caco-2 Cell Line

Name: Caco-2 Cell Line
Brand: Elabscience
SKU: EP-CL-0050
Availability: InStock

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Cell Name	Caco-2 Cell Line
Organism	Homo sapiens, Human
Age	72 years, male
Tissue	Colon
Morphology	Epithelial
Growth Properties	Adherent
Descriptions	Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Ref Caco-2 cells express retinoic acid binding protein I and retinol binding protein Ⅱ.
Biosafety Level	1

Complete Growth Medium	MEM(EP-CM-L0139)+20% FBS+1% P/S(EP-CM-L0048) (Caco-2 Cell Line complete medium (EP-ML-0050) is recommended.)
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio	1:2-1:4
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

Tumorigenic	Yes
Effects	Yes, in nude mice forms moderately well differentiated adenocarcinoma consistent with colonic primary(gradeⅡ).
Receptor Expression	This cell line expressed heat stable enterotoxin(Sta, E.coli) and epidermal growth factor(EGF).
Gene Expression	Keratin, Retinoic Acid Binding Protein 1, Retinol Binding Protein 2

EP-CM-P0139	MEM (with NEAA), powder
EP-CM-L0139	MEM (with NEAA)
EP-CM-L0048	Penicillin-Streptomycin Solution (100×)
EP-CM-L0006	Phosphate Buffer (PBS)
EP-CM-L0060	200mM L-Alanyl-L-Glutamine Solution (100×)
EP-CM-L0043	0.25% Trypsin Solution

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