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Enhanced Cell Counting Kit 8 (WST-8/CCK8)

  • Cat.No.:E-CK-A362

To Purchase E-CK-A362

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Detection principle

WST-8 is a compound similar to MTT, which can be reduced to orange formazan by some dehydrogenase in mitochondria in the presence of electron coupling reagent. The amount of formazan produced is directly proportional to the number of living cells. By measuring the absorbance at 450 nm, the amount of living cells can be calculated indirectly.

General Information

Application Viability/Proliferation/Cytotoxicity
Detection method Colorimetric Method
Sample type Cell samples
Detection instrument Microplate reader
Storage 2~8°C or -20°C , shading light
Shipping Ice bag
Expiration date 24 months

Citations

  1. LWT-FOOD SCIENCE AND TECHNOLOGY (2022) IF: 6.056
    Extraction, identification and anti-photoaging activity evaluation of collagen peptides from silver carp (Hypophthalmichthys molitrix) skin

    DOI: 10.1016/j.lwt.2022.114384

  2. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Regulating Th17/Treg Balance Contributes to the Therapeutic Effect of Ziyuglycoside I on Collagen-Induced Arthritis

    DOI: 10.3390/ijms232416105

  3. ACS Chemical Biology (2022) IF: 5.1
    YTHDF3 Facilitates eIF2AK2 and eIF3A Recruitment on mRNAs to Regulate Translational Processes in Oxaliplatin-Resistant Colorectal Cancer

    DOI: 10.1021/acschembio.2c00131

    PMID: 35708211

  4. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 5.875
    Safflower oil body nanoparticles deliver hFGF10 to hair follicles and reduce microinflammation to accelerate hair regeneration in androgenetic alopecia

    DOI: 10.1016/j.ijpharm.2022.121537

    PMID: 35150848

  5. ACTA PARASITOLOGICA (2021) IF: 1.44
    Evaluation of Allicin Against Alveolar Echinococcosis In Vitro and in a Mouse Model

    DOI: 10.1007/s11686-021-00434-z

    PMID: 34143400

  6. Current Medical Science (2022) IF: 2.135
    Downregulation of Astrocyte Elevated Gene-1 Expression Combined with All-Trans Retinoic Acid Inhibits Development of Vasculogenic Mimicry and Angiogenesis in Glioma

    DOI: 10.1007/s11596-022-2517-4

    PMID: 35201552

  7. JOURNAL OF CLINICAL LABORATORY ANALYSIS (2022) IF: 2.352
    Arctigenin inhibits apoptosis, extracellular matrix degradation, and inflammation in human nucleus pulposus cells by up-regulating miR-483-3p

    DOI: 10.1002/jcla.24508

    PMID: 35689566

  8. Experimental and Therapeutic Medicine (2022) IF: 2.447
    Verbascoside potentiates the effect of tyrosine kinase inhibitors on the induction of apoptosis and oxidative stress via the Abl-mediated MAPK signalling pathway in chronic myeloid leukaemia

    DOI: 10.3892/etm.2022.11441

    PMID: 35837042

  9. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism

    DOI: 10.3892/etm.2021.10407

  10. Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2022) IF: 2.464
    Anti-hepatic Fibrosis Potential of Ailanthone, A Quassinoid Isolated from Ailanthus altissima

    DOI: 10.1007/s43450-022-00340-z

  11. HEREDITAS (2022) IF: 2.595
    Arecoline promotes proliferation and migration of human HepG2 cells through activation of the PI3K/AKT/mTOR pathway

    DOI: 10.1186/s41065-022-00241-0

    PMID: 35836300

  12. MEDICAL SCIENCE MONITOR (2022) IF: 2.649
    Expression of Long Non-Coding RNA (lncRNA) SNHG5 in Patients with Refractory Diabetic Macular Edema and Its Regulatory Mechanism

    DOI: 10.12659/MSM.932996

  13. Computational and Mathematical Methods in Medicine (2022) IF: 2.809
    Apatinib Functioned as Tumor Suppressor of Synovial Sarcoma through Regulating miR-34a-5p/HOXA13 Axis

    DOI: 10.1155/2022/7214904

    PMID: 36276991

  14. Molecular Medicine Reports (2021) IF: 2.952
    FPR2 serves a role in recurrent spontaneous abortion by regulating trophoblast function via the PI3K/AKT signaling pathway

    DOI: 10.3892/mmr.2021.12478

    PMID: 34608500

  15. Eye and Vision (2022) IF: 3.257
    The ghrelin-GHSR-1a pathway inhibits high glucose-induced retinal angiogenesis in vitro by alleviating endoplasmic reticulum stress

    DOI: 10.1186/s40662-022-00291-5

    PMID: 35668539

  16. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.322
    IGF2BP3-NRF2 axis regulates ferroptosis in hepatocellular carcinoma

    DOI: 10.1016/j.bbrc.2022.08.040

    PMID: 36030651

  17. PHARMACEUTICAL BIOLOGY (2021) IF: 3.503
    Guizhi-Shaoyao-Zhimu decoction attenuates bone erosion in rats that have collagen-induced arthritis via modulating NF‐κB signalling to suppress osteoclastogenesis

    DOI: 10.1080/13880209.2021.1876100

  18. Cancer Management and Research (2022) IF: 3.602
    CSTF2 Acts as a Prognostic Marker Correlated with Immune Infiltration in Hepatocellular Carcinoma

    DOI: 10.2147/CMAR.S359545

    PMID: 36117731

  19. PLoS One (2023) IF: 3.752
    Inhibition of mycobacteria proliferation in macrophages by low cisplatin concentration through phosphorylated p53-related apoptosis pathway

    DOI: 10.1371/journal.pone.0281170

  20. JOURNAL OF PHARMACY AND PHARMACOLOGY (2022) IF: 3.765
    Lianhuaqingwen alleviates p53-mediated apoptosis in alveolar epithelial cells to prevent LPS-induced ALI

    DOI: 10.1093/jpp/rgac035

    PMID: 35640566

  21. PHYTOCHEMISTRY (2022) IF: 4.004
    Cytotoxic steroidal glycosides from the rhizomes of Paris polyphylla var. yunnanensis

    DOI: 10.1016/j.phytochem.2022.113577

  22. ONCOLOGY REPORTS (2023) IF: 4.136
    The PTBP1‑NCOA4 axis promotes ferroptosis in liver cancer cells

    DOI: 10.3892/or.2023.8482

  23. Human Cell (2022) IF: 4.174
    The α5-nAChR/PD-L1 axis facilitates lung adenocarcinoma cell migration and invasion

    DOI: 10.1007/s13577-022-00709-1

    PMID: 35593989

  24. MOLECULES (2021) IF: 4.412
    New Steroidal Saponins Isolated from the Rhizomes of Paris mairei

    DOI: 10.3390/molecules26216366

    PMID: 34770773

  25. MOLECULES (2022) IF: 4.412
    Mechanism of Action of Flavonoids of Oxytropis falcata on the Alleviation of Myocardial Ischemia–Reperfusion Injury

    DOI: 10.3390/molecules27051706

  26. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
    Discoidin Domain Receptors 1 Inhibition Alleviates Osteoarthritis via Enhancing Autophagy

    DOI: 10.3390/ijms21196991

    PMID: 32977456

    Sample: Cell Sample
  27. BMC CANCER (2022) IF: 4.638
    LAP3 contributes to IFN-γ-induced arginine depletion and malignant transformation of bovine mammary epithelial cells

    DOI: 10.1186/s12885-022-09963-w

    PMID: 35941558

  28. MOLECULES (2022) IF: 4.927
    Ajuforrestin A, an Abietane Diterpenoid from Ajuga ovalifolia var. calanthe, Induces A549 Cell Apoptosis by Targeting SHP2

    DOI: 10.3390/molecules27175469

    PMID: 36080236

  29. Scientific Reports (2023) IF: 4.996
    Long non-coding RNA KCNQ1OT1 alleviates postmenopausal osteoporosis by modulating miR-421-3p/mTOR axis

    DOI: 10.1038/s41598-023-29546-4

  30. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY (2022) IF: 5.085
    Adipose improves muscular atrophy caused by Sirtuin1 deficiency by promoting mitochondria synthesis

    DOI: 10.1016/j.biocel.2022.106246

    PMID: 35738524

  31. BIOORGANIC CHEMISTRY (2022) IF: 5.307
    Cytotoxic steroidal saponins from the rhizomes of Paris fargesii var. petiolata

    DOI: 10.1016/j.bioorg.2022.106305

  32. PHYTOMEDICINE (2022) IF: 5.34
    Protective role of casuarinin from Melastoma malabathricum against a mouse model of 5-fluorouracil–induced intestinal mucositis: Impact on inflammation and gut microbiota dysbiosis

    DOI: 10.1016/j.phymed.2022.154092

  33. CERAMICS INTERNATIONAL (2022) IF: 5.532
    Composite monetite/amorphous calcium phosphate bone cement promotes bone regeneration

    DOI: 10.1016/j.ceramint.2022.10.296

  34. Frontiers in Pharmacology (2021) IF: 5.811
    Shaoyao-Gancao Decoction Ameliorates the Inflammation State in Polycystic Ovary Syndrome Rats via Remodeling Gut Microbiota and Suppressing the TLR4/NF-κB Pathway

    DOI: 10.3389/fphar.2021.670054

    PMID: 34054541

    Sample: Cell Culture Supernatant
  35. PHYTOMEDICINE (2022) IF: 6.656
    Luteolin ameliorates hypoxia-induced pulmonary hypertension via regulating HIF-2α-Arg-NO axis and PI3K-AKT-eNOS-NO signaling pathway

    DOI: 10.1016/j.phymed.2022.154329

    PMID: 35843187

  36. Materials Today Bio (2021) IF: 7.348
    Cascade targeting codelivery of ingenol-3-angelate and doxorubicin for enhancing cancer chemoimmunotherapy through synergistic effects in prostate cancer

    DOI: 10.1016/j.mtbio.2021.100189

  37. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Efficacy of WWQ-131, a highly selective JAK2 inhibitor, in mouse models of myeloproliferative neoplasms

    DOI: 10.1016/j.biopha.2022.113884

    PMID: 36306591

  38. Frontiers in Immunology (2023) IF: 8.786
    Identification and validation of a prognostic signature of autophagy, apoptosis and pyroptosis-related genes for head and neck squamous cell carcinoma: to imply therapeutic choices of HPV negative patients

    DOI: 10.3389/fimmu.2022.1100417

  39. Frontiers in Immunology (2022) IF: 8.786
    AQP2 Promotes Astrocyte Activation by Modulating the TLR4/NFκB-p65 Pathway Following Intracerebral Hemorrhage.

    DOI: 10.3389/fimmu.2022.847360

  40. Materials Today Bio (2022) IF: 10.761
    Controllable graphene oxide-based biocompatible hybrid interface as an anti-fibrotic coating for metallic implants

    DOI: 10.1016/j.mtbio.2022.100326

    PMID: 35761844

  41. JOURNAL OF PINEAL RESEARCH (2022) IF: 12.081
    Melatonin alleviates the heat stress-induced impairment of Sertoli cells by reprogramming glucose metabolism

    DOI: 10.1111/jpi.12819

    PMID: 35906194

  42. JOURNAL OF HAZARDOUS MATERIALS (2022) IF: 14.224
    Polystyrene nanoparticle exposure supports ROS-NLRP3 axis-dependent DNA-NET to promote liver inflammation

    DOI: 10.1016/j.jhazmat.2022.129502

    PMID: 35868089

  43. Cellular & Molecular Immunology (2023) IF: 22.096
    HTR2A agonists play a therapeutic role by restricting ILC2 activation in papain-induced lung inflammation

    DOI: 10.1038/s41423-023-00982-6

    PMID: 36823235

  44. Molecular Cancer (2022) IF: 41.444
    CBX2 shapes chromatin accessibility promoting AML via p38 MAPK signaling pathway

    DOI: 10.1186/s12943-022-01603-y

    PMID: 35681235

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