HuH-7 Cell Line

Name: HuH-7 Cell Line
Brand: Elabscience
SKU: EP-CL-0120
Availability: InStock

Filter:

Size:

Price: Inquire

Qty:

Welcome to order from local distributors.

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Complete Growth Medium	DMEM(EP-CM-L0031)+10% FBS+1% P/S(EP-CM-L0048) (HuH-7 Cell Line complete medium (EP-ML-0120) is recommended.)
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio	1:2-1:4
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

EP-CM-P0031	DMEM (High Glucose), powder
EP-CM-L0031	DMEM (High glucose)
EP-CM-L0048	Penicillin-Streptomycin Solution (100×)
EP-CM-L0006	Phosphate Buffer (PBS)
EP-CM-L0060	200mM L-Alanyl-L-Glutamine Solution (100×)
EP-CM-L0043	0.25% Trypsin Solution

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

... Show All Show Less

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha