Yeast protein expression system is the most economical eukaryotic expression system for both secretion and intracellular expression. It is ideally suitable for large-scale production of recombinant eukaryotic proteins.
Elabscience's Yeast Protein Expression System Service start with gene analysis (S-CP-Y001), followed by subcloning (S-CP-Y002), screening of expression strains and small amount protein expression (S-CP-Y003). According to your project, we can offer the target protein in large scale (1~5L, S-CP-Y004 or 1~30L, S-CP-Y005). Results of each service will be delivered with our identification reports to keep you informed of the progress. You can order every single service or any combination of these services according to your project program.
Submit your project information and requirements and place your order, our experienced experts will help with your research and promise you the high quality services with competitive price.
Advantages
Proprietary Technologies: Our
advanced protein platform considers promoters, optimal copies of vector per
cell, inducibility, signal peptides, and cellular location, which are essential
for high-level recombinant protein production.
Humanized antibody production: Elabscience® has
developed a platform that produces humanized antibodies in yeast expression
system. The similarity between the yeast and mammalian system for recombinant
antibody expression enables Elabscience® to reduce the high cost
associated with manufacturing recombinant antibody in mammalian expression
system.
Streamlined Services: Elabscience® can take
your project directly from gene synthesis, to the downstream contents including
recombinant protein expression, antibody production, and assay development.
Yeast protein expression and purification services (Pichia pastoris System) Details
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Cat. No.
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Service Name
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Specification
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Timeline
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Delivery
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Quoted price
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S-CP-Y001
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Analysis of gene and target protein
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-
Codon optimization, protein hydrophilic,
hydrophobic, PI, structural domain, etc
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1-2 days
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Free
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S-CP-Y002
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Subcloning
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Gene synthesis
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Construction of expression vector
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Plasmids Prep and analysis
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3-4 weeks
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Expression vector containing the target gene
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Target sequencing report.
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E.coli containing plasmids of the expression vector
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Plasmid instructions
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$0.4/bp
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S-CP-Y003
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Screening of expression strains
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Amplification of recombinant plasmids and linear electrotransformation of Pichia pastor with the recombinant plasmids
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Obtain high-copy strain by antibiotic screening
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Small amount expression with ten selected positive
transformation strains and protein expression evaluation and optimization
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SDS-PAGE to identify the expression
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3-4 weeks
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SDS-PAGE analysis results
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Expression strain (extra charge, $1500 / strain)
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$800
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S-CP-Y004
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Protein expression and purification in large scale
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Large amount expression with selected strains of
high-expression
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Protein purification
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SDS-PAGE test
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2-3 weeks
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Purified recombinant protein (purity >90%)
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Related analysis reports
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$615
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S-CP-Y005
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High density cultivation of protein expression in
fermentation tanks and purification
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Carry out the experiment with the optimized
expression condition
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3-4 weeks
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Ultra large amount of purified protein (We have
fermentation tanks of 10L and 30L, thus high-purified protein up to gram
level can be delivered)
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Related analysis reports
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Inquire
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