Principle of Caspase Assay Kit (Colorimetric Method)
Caspase-specific peptide enzymes are linked to the yellow chromophore pNA (p-nitroanilide) as a substrate.Upon activation of Caspase, the enzyme cleaves the substrate, releasing the yellow chromophore. The absorbance of the released pNA can be measured using a spectrophotometer or enzyme reader at wavelengths around 405 nm or 400 nm, as pNA exhibits strong absorbance in this range.
Principle of Caspase Assay Kit (Colorimetric Method)
Advantages
- Multiple Choices:Provide a variety of Caspase assay kits to meet the different needs of customers
- Simple Operation:The composition of the reagent was optimized and the experimental operation was more time saving
- Cost-effective:The key components are developed in-house with reliable quality and more cost-effective price
- Wide Applications:The enzyme activity in samples can be detected quantitatively, and these kits is suitable for a variety of cells
Caspase Assay Kits Colorimetric Method
Principle of Caspase Assay Kit (Fluorescence Method)
Caspase-specific peptides are conjugated to high-affinity DNA fluorochrome as substrates. These substrates can penetrate the cell membrane and enter the cytoplasm. The substrates themselves are non-fluorescent and exhibit charge repulsion with DNA. Upon activation of Caspase, the enzyme cleaves the substrate, releasing the high-affinity DNA fluorochrome. This fluorochrome binds to DNA, resulting in strong fluorescence. This method allows for the detection of Caspase activity and observation of nuclear morphological changes during the process of apoptosis.
Caspase fluorescence method experimental principle
Advantages
- Easy to operate:One-step dyeing, saving time and effort
- Safe and non-toxic:Low cytotoxicity and did not affect the apoptotic process of cells
- Live-cell observation: Without lysis of the cells, live cell observations can be performed
- Wide Applications: Can be combined with multiple assay kits, choose diversification
Caspase Assay Kits (Fluorescence Method)
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