Caspase 2 Activity Assay Kit(Colorimetric Method)

Caspase 2, also known as Ich-1(ICE and CED-3 homolog 1) or Nedd-2, can be activated during signal transduction of apoptosis. Caspase 2 mRNA can be cleaved into two different forms. The mRNA product of full-length caspase 2 can be promote apoptosis, while the protein product of short mRNA can inhibit apoptosis. Caspase 2 can be activated by caspase 1, caspase 3 and granzyme B in vitro. This caspase 2 activity assay kit is based on caspase 2 that can catalyze the substrate Ac-VDQQD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 2 can be calculated by determining the absorbance at 405 nm.