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8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

Cat.No.:E-EL-0028
Reactivity: Universal

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes 8-OHdG in samples. No significant cross-reactivity or interference between 8-OHdG and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of 8-OHdG concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-OHdG. During the reaction, 8-OHdG in the sample or standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-OHdG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-OHdG in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 8-OHdG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 8-OHdG were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.79	10.11	46.98	4.48	9.61	48.89
Standard deviation	0.27	0.58	1.69	0.27	0.55	1.91
CV (%)	5.66	5.69	3.60	5.95	5.70	3.90

Recovery

The recovery of 8-OHdG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-100	91
EDTA plasma(n=8)	91-104	98
Cell culture media(n=8)	93-106	98

Linearity

Samples were spiked with high concentrations of 8-OHdG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Metabolism, Cardiovascular, Immunology, Cancer

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

NATURE (2020) IF: 42.779

DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25

DOI: 10.1038/s41586-020-2394-6
PMID: 32528174	Sample: DNA

Redox Biology (2021) IF: 11.799

Biomarkers of nucleic acid oxidation – a summary state-of-the-art

DOI: 10.1016/j.redox.2021.101872
PMID: 33579665

Cell Death & Disease (2022) IF: 9.685

Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease

DOI: 10.1038/s41419-022-05120-0
PMID: 35908070	Sample: Urine

ANTIOXIDANTS & REDOX SIGNALING (2021) IF: 8.401

Role of Nox4 in high calcium-induced renal oxidative stress damage and crystal deposition

DOI: 10.1089/ars.2020.8159
	Sample: kidney,NRK-52E cell

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 7.129

Ambient ozone, and urban PM2.5 co-exposure, aggravate allergic asthma via transient receptor potential vanilloid 1-mediated neurogenic inflammation

DOI: 10.1016/j.ecoenv.2022.114000
PMID: 35994908	Sample: lung

FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 7.376

New mechanism of nephrotoxicity of triptolide: Oxidative stress promotes cGAS-STING signaling pathway

DOI: 10.1016/j.freeradbiomed.2022.06.009
PMID: 35697291	Sample: HKC cell,HK2 cell

ANTIOXIDANTS & REDOX SIGNALING (2022) IF: 7.468

NEAT1 regulates CaOx crystal-induced renal tubular oxidative injury via miR-130/IRF1

DOI: 10.1089/ars.2022.0008
PMID: 36242511	Sample: HK-2 cells

Frontiers in Immunology (2022) IF: 7.561

Formation of Blood Neutrophil Extracellular Traps Increases the Mastitis Risk of Dairy Cows During the Transition Period

DOI: 10.3389/fimmu.2022.880578
PMID: 35572521	Sample: serum

PHARMACOLOGICAL RESEARCH (2022) IF: 7.658

Ginsenoside Rd ameliorates high glucose-induced retinal endothelial injury through AMPK-STRT1 interdependence

DOI: 10.1016/j.phrs.2022.106123
PMID: 35150861	Sample: Human umbilical vein endothelial cells (HUVECs)

Antioxidants (2022) IF: 7.675

Melatonin Prevents NaAsO2-Induced Developmental Cardiotoxicity in Zebrafish through Regulating Oxidative Stress and Apoptosis

DOI: 10.3390/antiox11071301
PMID: 35883792	Sample: embryos

Antioxidants (2022) IF: 7.675

Inhibition of Tumor Growth and Modulation of Antioxidant Activity of Rhodoxanthin Isolated from Taxus baccata Aril against B16F10 Murine Malignant Melanoma

DOI: 10.3390/antiox11112264
	Sample: plasma

Antioxidants (2022) IF: 7.675

Biological Effects of Hydrogen Water on Subjects with NAFLD: A Randomized, Placebo-Controlled Trial

DOI: 10.3390/antiox11101935
PMID: 36290657	Sample: plasma

Journal of Animal Science and Biotechnology (2022) IF: 6.175

Blood neutrophil extracellular traps: a novel target for the assessment of mammary health in transition dairy cows

DOI: 10.1186/s40104-022-00782-4
	Sample: serum

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208

Otoprotective Effects of Fucoidan Reduce Cisplatin-Induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells

DOI: 10.3390/ijms24043561
	Sample: UB/OC-2 cell

Antioxidants (2021) IF: 6.313

Antioxidant Effect of a Probiotic Product on a Model of Oxidative Stress Induced by High-Intensity and Duration Physical Exercise

DOI: 10.3390/antiox10020323
PMID: 33671691	Sample: serum

Frontiers in Immunology (2017) IF: 6.429

Chronic Obstructive Pulmonary Disease-Derived Circulating Cells Release IL-18 and IL-33 under Ultrafine Particulate Matter Exposure in a Caspase-1/8-Independent Manner

DOI: 10.3389/fimmu.2017.01415
	Sample: Cell culture supernatant

ENVIRONMENTAL RESEARCH (2021) IF: 6.498

Changes in genotoxicity, inflammatory and oxidative stress parameters of workers in marble processing plants

DOI: 10.1016/j.envres.2021.111209
PMID: 33895109	Sample: Plasma

Cancers (2022) IF: 6.639

The Polymorphisms of Genes Encoding Catalytic Antioxidant Proteins Modulate the Susceptibility and Progression of Testicular Germ Cell Tumor

DOI: 10.3390/cancers14041068
PMID: 35205816	Sample: plasma

Annals of the American Thoracic Society (2021) IF: 6.831

Association of Obstructive Sleep Apnea with the Aging Process

DOI: 10.1513/AnnalsATS.202007-771OC
PMID: 33662230	Sample: blood

ENVIRONMENTAL RESEARCH (2019) IF: 5.026

The impact on T-regulatory cell related immune responses in rural women exposed to polycyclic aromatic hydrocarbons (PAHs) in household air pollution in Gansu, China: A pilot investigation

DOI: 10.1016/j.envres.2019.03.053
PMID: 30951957	Sample: Urine

LIFE SCIENCES (2022) IF: 5.037

STING up-regulates VEGF expression in oxidative stress-induced senescence of retinal pigment epithelium via NF-κB/HIF-1α pathway

DOI: 10.1016/j.lfs.2021.120089
PMID: 35007563	Sample: ARPE-19 cell

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023) IF: 5.19

The toxicity of 4-tert-butylphenol in early development of zebrafish: morphological abnormality, cardiotoxicity, and hypopigmentation

DOI: 10.1007/s11356-023-25586-5
	Sample: embryos

JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195

Xiehuo Xiaoying decoction inhibits Tfh cell expansion and promotes Tfr cell amplification to ameliorate Graves’ disease

DOI: 10.1016/j.jep.2022.115826
	Sample: serum

AQUATIC TOXICOLOGY (2022) IF: 5.202

Spiromesifen conferred abnormal development in zebrafish embryos by inducing embryonic cytotoxicity via causing oxidative stress

DOI: 10.1016/j.aquatox.2022.106324
PMID: 36244087	Sample: embryos

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS (2022) IF: 5.589

A green-inspired method to prepare non-split high-density lipoprotein (HDL) carrier with anti-dysfunctional activities superior to reconstituted HDL

DOI: 10.1016/j.ejpb.2022.12.005
	Sample: Raw 264.7 cells

REDOX REPORT (2022) IF: 5.696

Atorvastatin-mediated downregulation of VCAM-1 and XO/UA/caspase 3 signaling averts oxidative damage and apoptosis induced by ovarian ischaemia/reperfusion injury

DOI: 10.1080/13510002.2022.2129192
PMID: 36200598	Sample: ovarian

ENVIRONMENTAL RESEARCH (2020) IF: 5.715

Effect of acute exposure of saxitoxin on development of zebrafish embryos (Danio rerio)

DOI: 10.1016/j.envres.2020.109432
PMID: 32247151	Sample: Tissue homogenate

Frontiers in Pharmacology (2022) IF: 5.811

Ursolic Acid Protects Neurons in Temporal Lobe Epilepsy and Cognitive Impairment by Repressing Inflammation and Oxidation.

DOI: 10.3389/fphar.2022.877898
	Sample: hippocampal tissue

Pharmaceuticals (2021) IF: 5.863

Fucoidan Protects against Acute Sulfoxaflor-Induced Hematological/Biochemical Alterations and Oxidative Stress in Male Mice

DOI: 10.3390/ph15010016
	Sample: serum

JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2020) IF: 4.018

Ovalbumin causes impairment of preimplantation embryonic growth in asthma-induced mice

DOI: 10.1016/j.jri.2020.103240
PMID: 33166807	Sample: Serum

CELL AND TISSUE RESEARCH (2022) IF: 4.051

Sestrin2 attenuates renal damage by regulating Hippo pathway in diabetic nephropathy

DOI: 10.1007/s00441-022-03668-z
PMID: 35821438	Sample: urine

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223

The involvement of oxidative stress, neuronal lesions, neurotransmission impairment, and neuroinflammation in acrylamide-induced neurotoxicity in C57/BL6 mice

DOI: 10.1007/s11356-021-18146-2
PMID: 35088269	Sample: brain

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223

3-Indolepropionic acid prevented chlorpyrifos-induced hepatorenal toxicities in rats by improving anti-inflammatory, antioxidant, and pro-apoptotic responses and abating DNA damage

DOI: 10.1007/s11356-022-21075-3
PMID: 35644820	Sample: Hepatic,renal

Journal of Clinical Medicine (2021) IF: 4.242

Cardiovascular Risk Factors and Their Relationship with Vascular Dysfunction in South African Children of African Ancestry

DOI: 10.3390/jcm10020354
PMID: 33477761	Sample: Urine

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Preservation of mitochondrial homeostasis is responsible for the ameliorative effects of Suhuang antitussive capsule on non-resolving inflammation via inhibition of NF-κB signaling and NLRP3 inflammasome activation

DOI: 10.1016/j.jep.2021.113827
PMID: 33460751	Sample: Cell culture supernatant(THP-1 cells)

Scientific Reports (2021) IF: 4.38

Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study

DOI: 10.1038/s41598-021-91256-6
PMID: 34083710	Sample: saliva

REDOX REPORT (2022) IF: 4.412

Suppression of glutathione system and upregulation of caspase 3-dependent apoptosis mediate rohypnol-induced gastric injury

DOI: 10.1080/13510002.2022.2074128
PMID: 35535549	Sample: gastric tissue

MOLECULES (2021) IF: 4.412

Ameliorative Potential of Hydroethanolic Leaf Extract of Parquetina nigrescens on d-Galactose-Induced Testicular Injury

DOI: 10.3390/molecules26113424
PMID: 34198754	Sample: testicular

JOURNAL OF CELLULAR PHYSIOLOGY (2019) IF: 4.522

Thioredoxin‐interacting protein deficiency alleviates phenotypic alterations of podocytes via inhibition of mTOR activation in diabetic nephropathy

DOI: 10.1002/jcp.28317
	Sample: Urine

Toxins (2021) IF: 4.546

The Protective Effect of Heme Oxygenase-1 on Liver Injury Caused by DON-Induced Oxidative Stress and Cytotoxicity

DOI: 10.3390/toxins13100732
PMID: 34679025	Sample: Hepa 1-6 Cell

TOXICOLOGY (2022) IF: 4.571

ATM deficiency aggravates the progression of liver fibrosis induced by carbon tetrachloride in mice

DOI: 10.1016/j.tox.2022.153397
	Sample: liver tissues

BIOFACTORS (2020) IF: 4.734

Berberine promotes peri‐implant osteogenesis in diabetic rats by ROS‐mediated IRS‐1 pathway

DOI: 10.1002/biof.1692
	Sample: serum

TRANSPLANTATION (2019) IF: 4.743

Hypothermic Oxygenated Machine Perfusion Alleviates Donation After Circulatory Death Liver Injury Through Regulating P-selectin-dependent and -independent Pathways in Mice

DOI: 10.1097/TP.0000000000002621
	Sample: Tissue homogenate

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2021) IF: 4.86

Indole-3-propionic acid mitigates chlorpyrifos-mediated neurotoxicity by modulating cholinergic and redox-regulatory systems, inflammatory stress, apoptotic responses and DNA damage in rats

DOI: 10.1016/j.etap.2021.103786
PMID: 34915193	Sample: brain

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 4.872

Nodularin induced oxidative stress contributes to developmental toxicity in zebrafish embryos

DOI: 10.1016/j.ecoenv.2020.110444
PMID: 32169726	Sample: Tissue homogenate

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 4.872

Dibutyl phthalate aggravated asthma-like symptoms through oxidative stress and increasing calcitonin gene-related peptide release

DOI: 10.1016/j.ecoenv.2020.110740
PMID: 32446102	Sample: Tissue homogenate

Oxidative Medicine and Cellular Longevity (2018) IF: 4.936

Losartan Ameliorates Calcium Oxalate-Induced Elevation of Stone-Related Proteins in Renal Tubular Cells by Inhibiting NADPH Oxidase and Oxidative Stress

DOI: 10.1155/2018/1271864
	Sample: NRK-52E cells

Journal of Personalized Medicine (2022) IF: 4.945

Interplay between Comprehensive Inflammation Indices and Redox Biomarkers in Testicular Germ-Cell Tumors

DOI: 10.3390/jpm12050833
PMID: 35629255	Sample: plasma

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY (2022) IF: 4.966

Possible mechanisms involved in the testicular-protective property of quercetin in rats exposed to endosulfan toxicity

DOI: 10.1016/j.pestbp.2022.105224
	Sample: testicular tissue

BUILDING AND ENVIRONMENT (2020) IF: 4.971

Winter vacation, indoor air pollution and respiratory health among rural college students: A case study in Gansu Province, China

DOI: 10.1016/j.buildenv.2020.107481
	Sample: Urine

TOXICON (2022) IF: 3.033

Caffeic acid protects against DNA damage, oxidative and inflammatory mediated toxicities, and upregulated caspases activation in the hepatorenal system of rats treated with aflatoxin B1

DOI: 10.1016/j.toxicon.2021.12.021
PMID: 34995555	Sample: liver,kidney

TOXICON (2022) IF: 3.035

Co-exposure to aflatoxin B1 and therapeutic coartem worsens hepatic and renal function through enhanced oxido-inflammatory responses and apoptosis in rats

DOI: 10.1016/j.toxicon.2022.106988
	Sample: liver，kidney

REPRODUCTIVE TOXICOLOGY (2022) IF: 3.143

Codeine alters female reproductive function by targeting ovarian steroidogenesis and folliculogenesis via the induction of oxidative stress, inflammation, and apoptosis

DOI: 10.1016/j.reprotox.2022.02.001
PMID: 35158039	Sample: Ovarian

Journal of Functional Foods (2017) IF: 3.144

Antioxidant and DNA protective effects of NTX, a proprietary glycyrrhizin/d-mannitol product, in association with alcohol consumption: A randomized, placebo-controlled, double-blind, crossover study

DOI: 10.1016/j.jff.2017.04.011
	Sample: testes

Animals (2022) IF: 3.231

Milk Pathogens in Correlation with Inflammatory, Oxidative and Nitrosative Stress Markers in Goat Subclinical Mastitis

DOI: 10.3390/ani12233245
	Sample: skimmed milk

Cardiovascular Toxicology (2022) IF: 3.239

Upregulation of Uric Acid Production and Caspase 3 Signalling Mediates Rohypnol-Induced Cardiorenal Damage

DOI: 10.1007/s12012-022-09723-z
PMID: 35103933	Sample: cardiac,renal

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2020) IF: 3.245

Possible differences in the mechanism of malignant transformation of HaCaT cells by arsenite and its dimethyl metabolites, particularly dimethylthioarsenics

DOI: 10.1016/j.jtemb.2020.126544
	Sample: Cell culture supernatant

ANALYTICAL BIOCHEMISTRY (2021) IF: 3.365

Potential contribution of ELISA and LFI assays to assessment of the oxidative stress condition based on 8-oxodG biomarker

DOI: 10.1016/j.ab.2021.114215
PMID: 33957135	Sample: Serum,Plasma,Saliva,Urine,Aqueous Homor,Tissue hom

ENVIRONMENTAL GEOCHEMISTRY AND HEALTH (2020) IF: 3.472

Blood lead and cadmium levels in occupationally exposed workers and their effect on markers of DNA damage and repair

DOI: 10.1007/s10653-020-00696-y
PMID: 32813162	Sample: Plasma

FUNGAL GENETICS AND BIOLOGY (2018) IF: 3.476

Mgm1 is required for maintenance of mitochondrial function and virulence in Candida albicans

DOI: 10.1016/j.fgb.2018.09.006

PSYCHIATRY AND CLINICAL NEUROSCIENCES (2019) IF: 3.489

Increased plasma levels of 8-oxoguanine DNA glycosylase-1 in bipolar disorder

DOI: 10.1111/pcn.12928
PMID: 31482650	Sample: Plasma

TOXICOLOGY LETTERS (2019) IF: 3.499

Apelin-13 reduces oxidative stress induced by uric acid via downregulation of renin-angiotensin system in adipose tissue

DOI: 10.1016/j.toxlet.2019.01.014
PMID: 30710622	Sample: Serum,Cell culture supernatant,Cell lysate

NEURAL PLASTICITY (2021) IF: 3.599

The Protective Role of E-64d in Hippocampal Excitotoxic Neuronal Injury Induced by Glutamate in HT22 Hippocampal Neuronal Cells

DOI: 10.1155/2021/7174287
PMID: 34721570	Sample: HT22 cell

LIFE SCIENCES (2020) IF: 3.647

Codeine exerts cardiorenal injury via upregulation of adenine deaminase/xanthine oxidase and caspase 3 signaling

DOI: 10.1016/j.lfs.2020.118717
PMID: 33159958	Sample: Serum,Plasma,Tissue homogenate

BIOLOGICAL TRACE ELEMENT RESEARCH (2021) IF: 3.738

Ecotoxicological Effects of Bimetallic PdNi/MWCNT and PdCu/MWCNT Nanoparticles onto DNA Damage and Oxidative Stress in Earthworms

DOI: 10.1007/s12011-021-02821-z
PMID: 34313947

Heliyon (2020) IF: 3.776

In vivo exposure to codeine induces reproductive toxicity: role of HER2 and p53/Bcl-2 signaling pathway

DOI: 10.1016/j.heliyon.2020.e05589
	Sample: testicular

JOURNAL OF KING SAUD UNIVERSITY SCIENCE (2022) IF: 3.829

Polyherbal formulation: The studies towards identification of composition and their biological activities

DOI: 10.1016/j.jksus.2022.102256
	Sample: Urine

Frontiers in Pharmacology (2019) IF: 3.845

AIM2 Inflammasome Activation Leads to IL-1α and TGF-β Release From Exacerbated Chronic Obstructive Pulmonary Disease-Derived Peripheral Blood Mononuclear Cells

DOI: 10.3389/fphar.2019.00257
PMID: 30930781	Sample: Cell culture supernatant

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2022) IF: 3.886

Fetal hyperhomocysteinemia is associated with placental inflammation and early breakdown of maternal-fetal tolerance in Pre-term birth

DOI: 10.1111/aji.13589
PMID: 35750632	Sample: serum

Toxins (2019) IF: 3.895

Tannic Acid Induces the Mitochondrial Pathway of Apoptosis and S Phase Arrest in Porcine Intestinal IPEC-J2 Cells

DOI: 10.3390/toxins11070397
	Sample: IPEC-J2 cells

NEUROTOXICITY RESEARCH (2021) IF: 3.911

Protective Effect of Chitosan Oligosaccharide against Hydrogen Peroxide-Mediated Oxidative Damage and Cell Apoptosis via Activating Nrf2/ARE Signaling Pathway

DOI: 10.1007/s12640-021-00419-w
PMID: 34622385	Sample: SH-SY5Y cell

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY (2021) IF: 2.043

Baicalein ameliorated cerebral ischemia-reperfusion injury dependent on calpain 1/AIF pathway

DOI: 10.1093/bbb/zbab222
	Sample: PC12 cell

ARTIFICIAL ORGANS (2018) IF: 2.111

Hydrogen Gas Does Not Ameliorate Renal Ischemia Reperfusion Injury in a Preclinical Model

DOI: 10.1111/aor.13118
	Sample: urine

REPRODUCTION FERTILITY AND DEVELOPMENT (2022) IF: 2.311

Capsaicin prevents radiotherapy-induced premature ovarian failure in rats

DOI: 10.1071/RD21235
PMID: 35101163	Sample: serum

PeerJ (2019) IF: 2.353

The role of serum and urinary biomarkers in the diagnosis of early diabetic nephropathy in patients with type 2 diabetes

DOI: 10.7717/peerj.7079
	Sample: urinary,serum

Metabolic Syndrome and Related Disorders (2022) IF: 2.363

Naringenin Prevents Inflammation, Apoptosis, and DNA Damage in Potassium Oxonate-Induced Hyperuricemia in Rat Liver Tissue: Roles of Cytochrome C, NF-κB, Caspase-3, and 8-Hydroxydeoxyguanosine

DOI: 10.1089/met.2022.0028
PMID: 35796694	Sample: Liver tissue

DRUG AND CHEMICAL TOXICOLOGY (2020) IF: 2.405

Effects of hyperbilirubinemia on markers of genotoxicity and total oxidant and antioxidant status in newborns

DOI: 10.1080/01480545.2019.1710182
	Sample: serum

ENVIRONMENTAL TOXICOLOGY (2018) IF: 2.491

The efficacy of oleuropein against non‐steroidal anti‐inflammatory drug induced toxicity in rat kidney

DOI: 10.1002/tox.22658
	Sample: Tissue homogenate

BMC Pharmacology & Toxicology (2022) IF: 2.605

Accidental lead in contaminated pipe-borne water and dietary furan intake perturbs rats’ hepatorenal function altering oxidative, inflammatory, and apoptotic balance

DOI: 10.1186/s40360-022-00615-0
	Sample: hepatic,renal

BIOLOGICAL TRACE ELEMENT RESEARCH (2020) IF: 2.639

Evaluation of DNA Damage and Expressions of DNA Repair Gene in Occupationally Lead Exposed Workers (Jodhpur, India)

DOI: 10.1007/s12011-020-02298-2
PMID: 32712906	Sample: Serum

ENVIRONMENTAL TOXICOLOGY (2019) IF: 2.649

Nano‐selenium attenuates nickel‐induced testosterone synthesis disturbance through inhibition of MAPK pathways in Sprague‐Dawley rats

DOI: 10.1002/tox.22768
	Sample: testicular tissues

ENVIRONMENTAL TOXICOLOGY (2019) IF: 2.649

Effects of phenanthrene on oxidative stress and inflammation in lung and liver of female rats

DOI: 10.1002/tox.22840
PMID: 31456356	Sample: Serum

Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65

San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

DOI: 10.1155/2022/7832540
	Sample: hepatic tissue

JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72

Kolaviron, a flavonoid-rich extract ameliorates busulfan-induced chemo-brain and testicular damage in male rats through inhibition of oxidative stress, inflammatory, and apoptotic pathways

DOI: 10.1111/jfbc.14071
PMID: 35060131	Sample: testis tissue

PLoS One (2020) IF: 2.74

Testicular toxicity following chronic codeine administration is via oxidative DNA damage and up-regulation of NO/TNF-α and caspase 3 activities

DOI: 10.1371/journal.pone.0224052
PMID: 32168344	Sample: Serum,Tissue homogenate

FUNDAMENTAL & CLINICAL PHARMACOLOGY (2022) IF: 2.747

The protective effect of 3-indolepropanoic acid on aflatoxin B1-induced systemic perturbation of the liver and kidney function in rats

DOI: 10.1111/fcp.12842
PMID: 36214208	Sample: liver,kidney

REDOX REPORT (2020) IF: 2.753

Codeine-induced sperm DNA damage is mediated predominantly by oxidative stress rather than apoptosis

DOI: 10.1080/13510002.2020.1752003
PMID: 32290793	Sample: Serum,Tissue homogenate

PLoS One (2017) IF: 2.806

A Comparative Study on Antioxidant System in Fish Hepatopancreas and Intestine Affected by Choline Deficiency: Different Change Patterns of Varied Antioxidant Enzyme Genes and Nrf2 Signaling Factors

DOI: 10.1371/journal.pone.0169888
	Sample: Tissue homogenate

EXCLI Journal (2020) IF: 2.837

Polygonum minus essential oil modulates cisplatin-induced hepatotoxicity through inflammatory and apoptotic pathways

DOI: 10.17179/excli2020-2355
PMID: 33122975	Sample: liver

Molecular Medicine Reports (2021) IF: 2.952

Remote ischemic post‑conditioning alleviates ischemia/reperfusion‑induced intestinal injury via the ERK signaling pathway‑mediated RAGE/HMGB axis

DOI: 10.3892/mmr.2021.12413
PMID: 34490475	Sample: serum

JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY (2020) IF: 1.016

Clinical Laboratory Manifestation and Molecular Diagnosis of β-Thalassemia Patients in Iraq

DOI: 10.1097/MPH.0000000000001656
	Sample: Serum

Revista da Associacao Medica Brasileira (2021) IF: 1.209

High serum 8-hydroxy-2′-deoxyguanosine levels predict DNA damage and aging in professional divers

DOI: 10.1590/1806-9282.20210748
	Sample: serum

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS (2020) IF: 1.28

Effects of grape juice consumption on muscle fatigue and oxidative stress in judo athletes: a randomized clinical trial

DOI: 10.1590/0001-3765202020191551
	Sample: plasma

MOLECULAR BIOLOGY REPORTS (2020) IF: 1.402

Codeine-induced hepatic injury is via oxido-inflammatory damage and caspase-3-mediated apoptosis

DOI: 10.1007/s11033-020-05983-6
PMID: 33211294	Sample: Hepatic

Systems Biology in Reproductive Medicine (2018) IF: 1.582

Grape seed procyanidins extract attenuates Cisplatin-induced oxidative stress and testosterone synthase inhibition in rat testes

DOI: 10.1080/19396368.2018.1450460
	Sample: serum

ANDROLOGIA (2018) IF: 1.588

LY294002, a PI3K pathway inhibitor, prevents leptin‐induced adverse effects on spermatozoa in Sprague‐Dawley rats

DOI: 10.1111/and.13196
	Sample: Tissue homogenate

JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662

Curcumin and Baicalin ameliorate ethanol‐induced liver oxidative damage via the Nrf2/HO‐1 pathway

DOI: 10.1111/jfbc.13425
	Sample: Serum

Archives of Environmental & Occupational Health (2021) IF: 1.663

A preliminary study on household air pollution exposure and health-related factors among rural housewives in Gansu province, northwest China

DOI: 10.1080/19338244.2021.1993775
PMID: 34748471	Sample: urine

Inorganic and Nano-Metal Chemistry (2022) IF: 1.716

Nephrotoxicity of nickel nano and microparticles in rat- a comparative, time dependent study with special reference to antioxidant defence system

DOI: 10.1080/24701556.2022.2048307
	Sample: urine

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS (2017) IF: 1.829

Mitochondrial DNA damage and oxidative damage in HL-60 cells exposed to 900 MHz radiofrequency fields

DOI: 10.1016/j.mrfmmm.2017.03.001
	Sample: HL-60 cell

Journal of Pediatric Urology (2021) IF: 1.83

The effect of hypothermia in a rat testicular torsion/detorsion model

DOI: 10.1016/j.jpurol.2021.01.045
PMID: 33610458	Sample: Serum,Tissue homogenate(testis)

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES (2022) IF: 1.885

Effect of oligosaccharides on the antioxidant, lipid and inflammatory profiles of rats with streptozotocin-induced diabetes mellitus

DOI: 10.1515/znc-2021-0215
	Sample: serum

Birth Defects Research (2020) IF: 1.896

Perinatal nicotine exposure alters lung development and induces HMGB1‐RAGE expression in neonatal mice

DOI: 10.1002/bdr2.1840
	Sample: Tissue homogenate

JOURNAL OF MEDICAL INVESTIGATION (2022)

Follicular fluid 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as biomarker for oxidative stress in intracytoplasmic sperm injection

DOI: 10.2152/jmi.69.112
	Sample: Follicular fluid (FF)

Meta Gene (2020)

Effect of exercise, synthetic anabolic steroids and protein intake on DNA damage in trained and untrained men

DOI: 10.1016/j.mgene.2020.100685
	Sample: Serum

International Journal of Reproductive Biomedicine (2021)

Association between a marker of sperm DNA damage and sperm indices in infertile males in Benin City, Nigeria: A cross-sectional study

DOI: 10.18502/ijrm.v19i2.8472
	Sample: Semen sample

Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2016) IF: 0.211

Sözlü Sunum Özetleri / Abstracts of Oral Presentations

DOI: 10.1515/tjb-2016-s409
	Sample: serum

TURKISH JOURNAL OF PEDIATRICS (2019) IF: 0.275

Impacts of phototherapy on DNA damage and total oxidant/ antioxidant status in jaundiced newborns.

DOI: 10.24953/turkjped.2019.05.008
	Sample: plasma

Journal of Essential Oil Bearing Plants (2019) IF: 0.6879

The Protective Effects of Thymol Against Ketoprofen Induced Damages on Pancreatic Acinar and Islet of Langerhans Cells in Rats

DOI: 10.1080/0972060X.2019.1625814
	Sample: Tissue homogenate

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Reviews/Q&A

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Reviews
Q&A

R****hSubmitted [ Oct 16 2022 ]

Asked: How to calculate the result

adminSubmitted [ Oct 16 2022 ]

Answered: Calculation of results Average the duplicate readings for each standard and samples. Plot a four-parameter logistic curve on log-log graph paper, with standard concentration on the x-axis and OD values on the y-axis. If the samples have been diluted, the concentration calculated from the standard curve must be multiplied by the dilution factor. If the OD of the sample is under the lowest limit of the standard curve, you should re-test it with an appropriate dilution. The actual concentration is the calculated concentration multiplied by the dilution factor. Please check the manual.(https://file.elabscience.com/Manual/elisa_kits/E-EL-0028.pdf)

M*********sSubmitted [ May 24 2021 ]

Asked: Is this kit useful for urine samples from rats?

adminSubmitted [ May 24 2021 ]

Answered: E-EL-0028 is suitable for the detection of rat urine samples. We have validated this kit with healthy rat urine, all the samples were undiluted and the reference value was about 2-12 ng/mL.

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