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FITC Anti-Human CD3 Antibody[OKT-3]

Uniprot : P07766
  • Cat.No.:E-AB-F1001C

  • Applications: FCM
  • Isotype: Mouse IgG2a, κ
  • Host: Mouse

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Antigen Details

Alternate Names T-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E
Swissprot P07766
References
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.

Product Details

Form Liquid
Clone No. OKT-3(See Other Available Formats)
Host Mouse
Isotype Mouse IgG2a, κ
Isotype Control FITC Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802C]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Reviews/Q&A

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  • Reviews
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Verified Customer

z*****cSubmitted [ Jun 13 2019 ]

  • Application:FCM
  • Species:Human
  • Sample:pbmc
  • Primary antibody:Undiluted

    30minutes

  • Temperature:4℃

  • Description:This antibody i used well helps me to finish my work
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Verified Customer

m*****oSubmitted [ Jun 13 2019 ]

  • Application:FCM
  • Species:Human
  • Sample:pbmc
  • Primary antibody:Undiluted

    30minutes

  • Temperature:4℃

  • Description:i can very quickly find the positive cells
Read More
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