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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Citations(68)

  • Cat.No.:E-BC-K235-M

  • Detection instrument: Microplate reader(500-520 nm)

Manual MSDS

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  • 48T
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Detection principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Performance characteristics

Synonyms ALT,GPT,Glutamic-pyruvic transaminase
Sample type Serum,plasma,animal tissue,cells,cell culture supernatant
Sensitivity 0.75 IU/L
Detection range 0.75-72.3 IU/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 80 min
Precision Average inter-assay CV: 9%Average intra-assay CV: 5.300%
Other instruments required Micropipettor, Vortex mixer, Incubator, Multichannel pipette
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

No significant change of ALT activity was observed, the LCFG nanoparticles did not include hepatotoxicity.

X Zhang et al investigate the usage of H2O2 and O2 for cooperative chemodynamic/starvation cancer therapy. Alanine aminotransferase (ALT) avtivity in mouse blood was determined using ALT activity assay kit (E-BC-K235-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.75-72.3 IU/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Porcine serum

1

Rat serum

1

10% Rat brain tissue homogenization

1

10% Rat heart tissue homogenization

1

10% Rat liver tissue homogenization

40-60

10% Rat kidney tissue homogenization

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Atorvastatin Ester Regulates Lipid Metabolism in Hyperlipidemia Rats via the PPAR-signaling Pathway and HMGCR Expression in the Liver

    DOI: 10.3390/ijms222011107

    PMID: 34681767

    		 Sample: Serum
  2. Frontiers in Immunology (2019) IF: 4.716
    Gal-3 Deficiency Suppresses Novosphyngobium aromaticivorans Inflammasome Activation and IL-17 Driven Autoimmune Cholangitis in Mice

    DOI: 10.3389/fimmu.2019.01309
    Sample: Serum
  3. Frontiers in Pharmacology (2022) IF: 5.811
    Uncovering the Pharmacological Mechanisms of Gexia-Zhuyu Formula (GXZY) in Treating Liver Cirrhosis by an Integrative Pharmacology Strategy.

    DOI: 10.3389/fphar.2022.793888
    Sample: Serum
  4. FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
    Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1

    DOI: 10.1016/j.fct.2021.112124

    PMID: 33727180

    		 Sample: Serum
  5. ACS Applied Bio Materials (2020)
    Noninvasive Visualization of Obesity-Boosted Inflammation in Orthotopic Pancreatic Ductal Adenocarcinoma Using an Octapod Iron Oxide Nanoparticle

    DOI: 10.1021/acsabm.0c00841
    Sample: Serum
  6. Toxicology Reports (2022)
    Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats

    DOI: 10.1016/j.toxrep.2022.06.005
    Sample: Plasma
  7. Open Life Sciences (2022) IF: 1.311
    β-Hydroxybutyrate upregulates FGF21 expression through inhibition of histone deacetylases in hepatocytes

    DOI: 10.1515/biol-2022-0095
    Sample: Serum
  8. Journal of Oleo Science (2022) IF: 1.628
    Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

    DOI: 10.5650/jos.ess22033

    PMID: 35965085

    		 Sample: Liver
  9. DRUG DEVELOPMENT RESEARCH (2019) IF: 1.742
    Montelukast modifies simvastatin‐induced myopathy and hepatotoxicity

    DOI: 10.1002/ddr.21581
    Sample: Liver
  10. Molecular Medicine Reports (2020) IF: 2.1
    Pretreatment with the ALDH2 activator Alda‑1 protects rat livers from ischemia/reperfusion injury by inducing autophagy

    DOI: 10.3892/mmr.2020.11312

    PMID: 32705206

    		 Sample: Serum
  11. JOURNAL OF TOXICOLOGICAL SCIENCES (2022) IF: 2.196
    Dexmedetomidine attenuates acute stress-induced liver injury in rats by regulating the miR-34a-5p/ROS/JNK/p38 signaling pathway

    DOI: 10.2131/jts.47.169

    PMID: 35527005

    		 Sample: Serum
  12. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Exogenous hydrogen sulfide protects against hepatic ischemia/reperfusion injury by inhibiting endoplasmic reticulum stress and cell apoptosis

    DOI: 10.3892/etm.2021.10231

    PMID: 34093755

    		 Sample: Serum
  13. Veterinary Sciences (2023) IF: 2.518
    Effect of Feeding Pomegranate (Punica granatum) Peel and Garlic (Allium sativum) on Antioxidant Status and Reproductive Efficiency of Female Rabbits

    DOI: 10.3390/vetsci10030179
    Sample: Serum
  14. Dose-Response (2022) IF: 2.623
    Biochemical Investigation of Therapeutic Potentials of Plant-Based Bioactive Compounds as Stimulators of Glucagon like peptide-1 Secretion :

    DOI: 10.1177/15593258221114184
    Sample: Serum
  15. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

    DOI: 10.1155/2022/7832540
    Sample: Hepatic Tissue,Serum
  16. JOURNAL OF SEPARATION SCIENCE (2020) IF: 2.878
    High‐throughput metabolomics method based on liquid chromatography‐mass spectrometry: insights into the underlying mechanisms of salinity‐alkalinity exposures‐induced metabolites changes in Barbus capito

    DOI: 10.1002/jssc.202000861
    Sample: Serum
  17. BIOSCIENCE REPORTS (2018) IF: 2.899
    Sterol 27-hydroxylase gene dosage and the antiatherosclerotic effect of Rifampicin in mice

    DOI: 10.1042/BSR20171162
    Sample: Plasma
  18. PeerJ (2022) IF: 2.984
    3,4-dihydroxyphenylethyl alcohol glycoside reduces acetaminophen-induced acute liver failure in mice by inhibiting hepatocyte ferroptosis and pyroptosis

    DOI: 10.7717/peerj.13082

    PMID: 35310165

    		 Sample: Plasma
  19. Journal of Nanomaterials (2021) IF: 2.986
    Effect of Hafnium Coating on Osseointegration of Titanium Implants: A Split Mouth Animal Study

    DOI: 10.1155/2021/7512957
    Sample: Mandibular Bone
  20. NEUROSCIENCE LETTERS (2021) IF: 3.046
    Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

    DOI: 10.1016/j.neulet.2021.136385

    PMID: 34871743

    		 Sample: Serum
  21. FEBS LETTERS (2020) IF: 3.057
    Islet‐cell autoantigen 69 accelerates liver regeneration by downregulating Tgfbr1 and attenuating Tgfβ signaling in mice

    DOI: 10.1002/1873-3468.13859
    Sample: Serum
  22. Frontiers in Physiology (2019) IF: 3.201
    Dietary Bile Acids Enhance Growth, and Alleviate Hepatic Fibrosis Induced by a High Starch Diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 Pathway in Micropterus salmoides

    DOI: 10.3389/fphys.2019.01430

    PMID: 31824338

    		 Sample: Plasma
  23. International Journal of Environmental Research and Public Health (2022) IF: 3.39
    Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners

    DOI: 10.3390/ijerph19010498

    PMID: 35010758

    		 Sample: Plasma
  24. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2022) IF: 3.568
    Myrrhone inhibits the progression of hepatic fibrosis by regulating the abnormal activation of hepatic stellate cells

    DOI: 10.1002/jbt.23177

    PMID: 35983967

    		 Sample: Serum
  25. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

    DOI: 10.1007/s11011-022-00952-3

    PMID: 35298730

    		 Sample: Serum
  26. LIFE SCIENCES (2020) IF: 3.647
    Pretreatment of exosomes derived from hUCMSCs with TNF-α ameliorates acute liver failure by inhibiting the activation of NLRP3 in macrophage

    DOI: 10.1016/j.lfs.2020.117401

    PMID: 32035931

    		 Sample: Whole Blood,Tissue Homogenate
  27. BMC Complementary and Alternative Medicine (2022) IF: 3.659
    Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues

    DOI: 10.1186/s12906-022-03536-x

    PMID: 35277165

    		 Sample: Serum
  28. American Journal of Translational Research (2021) IF: 4.06
    Personalized nursing improves physical condition and life quality of patients undergoing interventional therapy for liver cancer

    PMID: 35035768

    		 Sample: Blood
  29. JOURNAL OF INORGANIC BIOCHEMISTRY (2021) IF: 4.155
    In vitro and in vivoactivity of series of cationic dinuclearPt(II) complexes

    DOI: 10.1016/j.jinorgbio.2021.111619

    PMID: 34597886

    		 Sample: Serum
  30. Food Bioscience (2022) IF: 4.24
    Fatty acids of Pleurotus florida mushroom: Potential molecules for blood glucose control

    DOI: 10.1016/j.fbio.2022.101558
    Sample: Blood
  31. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    		 Sample: Serum
  32. DALTON TRANSACTIONS (2021) IF: 4.39
    A new bis-pyrazolylpyridine ruthenium(III) complex as a potential anticancer drug: in vitro and in vivo activity in murine colon cancer

    DOI: 10.1039/D1DT00185J

    PMID: 33982702

    		 Sample: Serum
  33. Journal of Functional Foods (2021) IF: 4.451
    Heracleum dissectum Ledeb. ethanol extract attenuates metabolic syndrome symptoms in high-fat diet-induced obese mice by activating adiponectin/AMPK signaling

    DOI: 10.1016/j.jff.2021.104581
    Sample: Blood
  34. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
    Mechanistic insights into the effects of SREBP1c on hepatic stellate cell and liver fibrosis

    DOI: 10.1111/jcmm.15614

    PMID: 32678475

    		 Sample: Serum
  35. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
    Isoliquiritigenin ameliorates caerulein‐induced chronic pancreatitis by inhibiting the activation of PSCs and pancreatic infiltration of macrophages

    DOI: 10.1111/jcmm.15498
    Sample: Serum
  36. AMERICAN JOURNAL OF CHINESE MEDICINE (2021) IF: 4.667
    Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice

    DOI: 10.1142/S0192415X21500452

    PMID: 33827384

    		 Sample: Serum
  37. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2021) IF: 4.86
    Apoptosis is induced by sub-acute exposure to 3-MCPD and glycidol on Wistar Albino rat brain cells

    DOI: 10.1016/j.etap.2021.103735

    PMID: 34461274

    		 Sample: Blood
  38. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Oridonin regulates the polarized state of Kupffer cells to alleviate nonalcoholic fatty liver disease through ROS-NF-κB

    DOI: 10.1016/j.intimp.2021.108290

    PMID: 34717194

    		 Sample: Serum
  39. LIFE SCIENCES (2022) IF: 5.037
    Aloe emodin 3-O-glucoside inhibits cell growth and migration and induces apoptosis of non-small-cell lung cancer cells via suppressing MEK/ERK and Akt signalling pathways

    DOI: 10.1016/j.lfs.2022.120495

    PMID: 35341826

    		 Sample: Serum
  40. Viruses-Basel (2021) IF: 5.048
    Favipiravir Inhibits Mayaro Virus Infection in Mice

    DOI: 10.3390/v13112213

    PMID: 34835018

    		 Sample: Serum
  41. MOLECULAR NUTRITION & FOOD RESEARCH (2020) IF: 5.309
    Gymnaster Koraiensis Extract Alleviated Metabolic Syndrome Symptoms and Stimulated UCP1‐Independent Energy Consumption via AMPK Activation in White Adipose Tissue

    DOI: 10.1002/mnfr.202000490
    Sample: Serum
  42. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: Serum
  43. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    		 Sample: Serum
  44. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Glabridin inhibits liver fibrosis and hepatic stellate cells activation through suppression of inflammation and oxidative stress by activating PPARγ in carbon tetrachloride-treated mice

    DOI: 10.1016/j.intimp.2022.109433
    Sample: Serum
  45. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    A novel reactive aldehyde species inhibitor prevents the deleterious effects of ethanol in an animal model of alcoholic liver disease

    DOI: 10.1016/j.intimp.2022.109400
    Sample: Serum
  46. Frontiers in Pharmacology (2021) IF: 5.811
    Metformin Alleviates Steatohepatitis in Diet-Induced Obese Mice in a SIRT1-Dependent Way

    DOI: 10.3389/fphar.2021.704112

    PMID: 34483906

    		 Sample: Liver
  47. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924
    Pyruvate Upregulates Hepatic FGF21 Expression by Activating PDE and Inhibiting cAMP–Epac–CREB Signaling Pathway

    DOI: 10.3390/ijms23105490
    Sample: Serum
  48. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Apolipoprotein A-IV Has Bi-Functional Actions in Alcoholic Hepatitis by Regulating Hepatocyte Injury and Immune Cell Infiltration

    DOI: 10.3390/ijms24010670
    Sample: Aml-12 Cell
  49. PHYTOTHERAPY RESEARCH (2022) IF: 6.388
    Resveratrol reverses TGF-β1-mediated invasion and metastasis of breast cancer cells via the SIRT3/AMPK/autophagy signal axis

    DOI: 10.1002/ptr.7608

    PMID: 36086852

    		 Sample: Serum
  50. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Activation of α7nAChR preserves intestinal barrier integrity by enhancing the HO-1 / STAT3 signaling to inhibit NF-κB activation in mice

    DOI: 10.1016/j.biopha.2022.112733

    PMID: 36068769

    		 Sample: Serum
  51. Cells (2021) IF: 6.6
    Indole-3-Carboxaldehyde Restores Gut Mucosal Integrity and Protects from Liver Fibrosis in Murine Sclerosing Cholangitis

    DOI: 10.3390/cells10071622

    PMID: 34209524

    		 Sample: Serum
  52. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2023) IF: 6.633
    Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ

    DOI: 10.1016/j.bbadis.2023.166643
    Sample: Serum
  53. PLoS Pathogens (2021) IF: 6.823
    IRF3-mediated pathogenicity in a murine model of human hepatitis A

    DOI: 10.1371/journal.ppat.1009960

    PMID: 34591933

    		 Sample: Serum
  54. PHARMACOLOGICAL RESEARCH (2019) IF: 7.658
    Dasatinib ameliorates chronic pancreatitis induced by caerulein via anti-fibrotic and anti-inflammatory mechanism

    DOI: 10.1016/j.phrs.2019.104357

    PMID: 31356863

    		 Sample: Serum
  55. Antioxidants (2023) IF: 7.675
    Bone Marrow Mesenchymal Stem-Cell-Derived Exosomes Ameliorate Deoxynivalenol-Induced Mice Liver Damage

    DOI: 10.3390/antiox12030588
    Sample: Serum
  56. mBio (2021) IF: 7.867
    Ileal Bile Acid Transporter Inhibitor Improves Hepatic Steatosis by Ameliorating Gut Microbiota Dysbiosis in NAFLD Model Mice | mBio

    DOI: 10.1128/mBio.01155-21
    Sample: Serum
  57. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoids

    DOI: 10.1016/j.scitotenv.2021.150328

    PMID: 34571217

    		 Sample: Liver Organoids (Los)
  58. SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 7.963
    Chronic exposure to polyvinyl chloride microplastics induces liver injury and gut microbiota dysbiosis based on the integration of liver transcriptome profiles and full-length 16S rRNA sequencing data

    DOI: 10.1016/j.scitotenv.2022.155984

    PMID: 35588832

    		 Sample: Serum
  59. Cellular and Molecular Gastroenterology and Hepatology (2022) IF: 8.797
    DNA 6mA Demethylase ALKBH1 Orchestrates Fatty Acid Metabolism and Suppresses Diet-induced Hepatic Steatosis

    DOI: 10.1016/j.jcmgh.2022.08.011

    PMID: 36058506

    		 Sample: Serum
  60. Acta Biomaterialia (2021) IF: 8.947
    An efficient and safe MUC1-dendritic cell-derived exosome conjugate vaccine elicits potent cellular and humoral immunity and tumor inhibition in vivo

    DOI: 10.1016/j.actbio.2021.10.041

    PMID: 34757230

    		 Sample: Serum
  61. ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022) IF: 9.028
    Lipid Metabolic Disorder Induced by Pyrethroids in Nonalcoholic Fatty Liver Disease of Xenopus laevis

    DOI: 10.1021/acs.est.2c00516
    Sample: Plasma
  62. JOURNAL OF HAZARDOUS MATERIALS (2020) IF: 9.038
    The combined adverse effects of cis-bifenthrin and graphene oxide on lipid homeostasis in Xenopus laevis

    DOI: 10.1016/j.jhazmat.2020.124876

    PMID: 33360192
  63. ACS Applied Materials & Interfaces (2021) IF: 9.229
    Synergistic Multimodal Cancer Therapy Using Glucose Oxidase@CuS Nanocomposites

    DOI: 10.1021/acsami.1c12235

    PMID: 34448397

    		 Sample: Plasma
  64. Cell Reports (2022) IF: 9.995
    Human hepatocyte PNPLA3-148M exacerbates rapid non-alcoholic fatty liver disease development in chimeric mice

    DOI: 10.1016/j.celrep.2022.111321

    PMID: 36103835

    		 Sample: Serum
  65. Molecular Therapy-Nucleic Acids (2022) IF: 10.183
    Chemically modified in-vitro-transcribed mRNA encoding thrombopoietin stimulates thrombopoiesis in mice

    DOI: 10.1016/j.omtn.2022.08.017

    PMID: 36090760

    		 Sample: Plasma
  66. HEPATOLOGY (2018) IF: 14.079
    MicroRNA 15a/16‐1 suppresses aryl hydrocarbon receptor–dependent interleukin‐22 secretion in CD4+ T cells and contributes to immune‐mediated organ injury

    DOI: 10.1002/hep.29573
    Sample: Serum
  67. Advanced Science (2023) IF: 17.521
    Phosphomevalonate Kinase Controls β-Catenin Signaling via the Metabolite 5-Diphosphomevalonate

    DOI: 10.1002/advs.202204909

    PMID: 36808719

    		 Sample: Serum
  68. Nature Communications (2022) IF: 17.694
    Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

    DOI: 10.1038/s41467-022-35481-1

    PMID: 36572670

    		 Sample: Serum
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A

Verified Customer

a****gSubmitted [ May 29 2019 ]

  • Application:Biochemical
  • Description:This alanine aminotransferase assay kit requires a small sample size, which is of great help to our real project.
Read More
Read Less
Q

Y**NSubmitted [ Sep 22 2023 ]

Asked: Hi, I am using a kit from another company to measure ALT in HepG2. But I\'m worried because Normal is measured too high. Are there any cases where Normal is high when using this kit?

Reply

A

adminSubmitted [ Sep 22 2023 ]

Answered: Hi Yoon, This kit can detect HepG2 samples, and the following is a reference for laboratory result : ALT activity of HepG2：3.659U/gprot.

Q

y**gSubmitted [ Aug 04 2023 ]

Asked: How to collect cell culture supernatant and perform subsequent detection experiments, and what is the difference between supernatant and direct use of cell lysis buffer?

Reply

A

adminSubmitted [ Aug 04 2023 ]

Answered: 1. The preparation of cell culture supernatant: Suspension cells: Centrifuge the cell suspension 1000g for 10 minutes, and take the supernatant on ice for detection. Adherent cells: directly absorb the supernatant, centrifuge 1000g for 10 minutes, and place on ice for detection. 2. The cell supernatant reflects the enzyme activity on the outside of the cell, while the cells treated with the cell lysis buffer reflect the enzyme activity inside the cell.

Q

E**************zSubmitted [ May 15 2023 ]

Asked: We are currently using C57BL/6 strain and the maximum serum that we collect is only 30 uL. Can you tell me how much serum sample that I have to use for one experiment?

Reply

A

adminSubmitted [ May 15 2023 ]

Answered: The minimum requirement for a single well is 10 uL for kit E-BC-K235-M

Q

M*****aSubmitted [ Aug 31 2022 ]

Asked: Will it affect result if this kit is at room temperature for half a month？

Reply

A

adminSubmitted [ Aug 31 2022 ]

Answered: No, it will not influence the result. Please ensure using.

Q

M*****aSubmitted [ Aug 22 2022 ]

Asked: Can you please help me understand exactly what a carmen unit is and why we are using it instead of the conventional method of plotting a standard curve of concentration of standard vs the absorbance. How is the carmen unit relevant to this exact calculation I would also like to know how you arrived at the numbers 0, 28, 57, 97, 150, 200 for the standard Carmen values.

Reply

A

adminSubmitted [ Aug 22 2022 ]

Answered: 1. This indicator is special, and the method used is the Reitman-Frankel method. The standard curve of the Reitman-Frankel method is determined by comparing the colorimetric method experimental result with the result of karmen spectrophotometry (rate method), and the report result of Karmen units will be more accurate. 2. The Karmen's units corresponding to the standard are fixed and converted according to the concentration of pyruvate.

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