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Cat.No.:E-AB-F1216UC
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Alternate Names | Mouse major histocompatibility complex (MHC) H-2, MHC I |
References |
1. Stallcup KC, et al. 1981. J. Immunol. 127:923.
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Form | Liquid |
Clone No. | M1/42(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | FITC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833C] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | FITC |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |