IDA Focurose 6FF can be used for separation and purification with the interactions between chelated metal ion and certain amino acids (mainly include histidine, cysteine, tryptophan) on the side chain of protein, and it is suitable for separation and purification of His-tagged protein and biological molecules which interact with metal ion.

Product Features

1. Quick and easy (one-step purification).
2. Wide application, simple-operation. Suitable for gravity column and prepacked column.
3. Multiple selection. Various metal ions (e.g. Cu2+, Zn2+, Fe2+, Co2+, Ca2+, etc.) can be used.

Product Datasheet

Matrix	Highly cross-linked 6% agarose
Particle size range	45-165 µm
Average particle size	90 µm
Binding capacity	34 µM Cu2+/mL(media)
Media type	Affinity Chromatography Medium
pH stability	3-12(long-term), 2-14(short term)
Chemical stability	All of the commonly used solution. Avoid of chelating agent(e.g. EDTA, EGTA) and reductant(e.g. DTT, DTE).
Maximum flow rate	300-600 cm/h
Pressure	≤ 0.3 Mpa
Storage buffer	20% Ethanol
Storage temperature	4~30℃(4~8℃ is preferred)

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Cat. No	E-CM-AF22
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