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Cat.No.:E-BC-K131-M
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Protocols for metabolism assays
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Sample type | Animal tissue |
Sensitivity | 3.94 U/mL |
Detection range | 12.5-2000 U/mL. |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 60 min |
Precision | Average inter-assay CV: 8.500%Average intra-assay CV: 4.500% |
Other instruments required | Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
The activity of lactase in pig jejunum was significantly changed by different daily diet.
H M J Hees et al investigate the supplementation of oat hulls in piglets colonic microbiota development. Activity of lactase in pig jejunum was determined using lactase activity assay kit (E-BC-K131-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (12.5-2000 U/mL).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
10% Rat lleal tissue homogenate |
1 |
10% Rat jejunum tissue homogenate |
1 |
10% Rat liver tissue homogenate |
1 |
10% Rat kidney tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl).
PMID: 34198013 |
Sample: duodenal |
Sample: jejunum |