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Cat.No.:E-BC-K025-S
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Synonyms | MDA |
Sample type | Serum,plasma,animal tissue |
Sensitivity | 0.38 nmol/mL |
Detection range | 0.38-133.33 nmol/mL |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 90 min |
Precision | Average inter-assay CV: 8%Average intra-assay CV: 4.900% |
Other instruments required | Micropipettor, Vortex mixer, Incubator, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol |
Storage | 2-8℃ |
Valid period | 12 months |
The level of MDA was significantly increased (*P<0.005) in cells treating with the nanoreactor.
XX Yang et al investigates the function of a nanoreactor that can induce ferroptosis of tumor cells by releasing DHA and iron. Malondialdehyde (MDA) of Lewis lung cancer cells was determined using MDA colorimetric assay kit (E-BC-K025-S).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (0.38-133.33 nmol/mL).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human plasma |
1 |
Rat serum |
1 |
Rat plasma |
1 |
Mouse serum |
1 |
Mouse plasma |
1 |
10% Rat heart tissue homogenate |
1 |
10% Rat liver tissue homogenate |
1 |
10% Rat spleen tissue homogenate |
1 |
10% Rat lung tissue homogenate |
1 |
10% Rat kidney tissue homogenate |
1 |
10% Rat brain tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
PMID: 35031271 |
Sample: Lung |
PMID: 35489465 |
Sample: Pig Testicular Cell (St Cell) |
Sample: Acute Myeloid Leukemia (Aml) Cell |
Sample: Liver |
PMID: 34769040 |
Sample: Serum |
Sample: Serum |
DOI: 10.3390/ph16010109 |
|
Sample: Liver Tissue,Hepg2 Cell |
Sample: Aortas |
DOI: 10.1155/2022/5631488 |
|
Sample: Aortic Tissue |
Sample: Plasma |
DOI: 10.1155/2020/9046862 |
|
PMID: 32318290 |
Sample: Serum |
Sample: Plasma |
Sample: Serum,Liver,Skin |
Sample: Thyroid Gland,Liver |
PMID: 32796257 |
Sample: Serum |
DOI: 10.1111/jfbc.13371 |
|
Sample: Tissue Homogenate |
Sample: Plasma |
DOI: 10.1002/ddr.21581 |
|
Sample: Liver |
PMID: 33567918 |
Sample: Serum |
Sample: Serum |
PMID: 31346790 |
Sample: Blood,Urine |
Sample: Tissue Homogenate |
Sample: Sh-Sy5Y Cell |
Sample: Lung |
PMID: 35389159 |
Sample: Serum |
DOI: 10.1155/2021/2073296 |
|
Sample: Beas-2B Cell |
Sample: Hippocampal Tissue |
PMID: 36252429 |
Sample: Sk-N-Sh Cells |
Sample: Serum |
Sample: Tissue Homogenate(Jejunum) |
DOI: 10.1002/jez.2603 |
|
Sample: Brain |
DOI: 10.1111/jfbc.14129 |
|
PMID: 35298033 |
Sample: Gastric,Jejunal, Colonic |
DOI: 10.1111/fcp.12845 |
|
Sample: Renal |
DOI: 10.1111/and.14028 |
|
PMID: 33650701 |
Sample: Tissue Homogenate(Testicular) |
PMID: 32689984 |
Sample: Plant Fluid |
PMID: 32550799 |
Sample: Serum |
DOI: 10.3390/cimb44110358 |
|
PMID: 36354670 |
Sample: Serum |
DOI: 10.3390/cimb45020057 |
|
Sample: Colonic Tissue |
Sample: Tissue Homogenate |
DOI: 10.3390/ani13030363 |
|
Sample: Plasma |
PMID: 34705867 |
Sample: Jejunal Mucosa,Blood |
DOI: 10.1155/2022/9943598 |
|
PMID: 35818443 |
Sample: Hippocampal Tissue |
DOI: 10.3390/pr10101985 |
|
Sample: Gastric Tissue |
DOI: 10.3390/pr10071314 |
|
Sample: Stomach |
PMID: 35252419 |
Sample: Ovarian |
PMID: 35635784 |
Sample: Brain Tissue |
Sample: Blood |
PMID: 35751788 |
Sample: Hippocampal Neurons |
PMID: 36152772 |
Sample: Brain Tissue |
DOI: 10.21037/atm-22-483 |
|
PMID: 35280364 |
Sample: Myocardium |
PMID: 33383446 |
Sample: Lung Tissue |
PMID: 33370579 |
Sample: Sk-N-Sh Cell,Imr-32 Cell |
Sample: Kidney Tissue |
Sample: Testicular Tissue |
DOI: 10.1002/tox.23639 |
|
Sample: Serum,Nr8383 Cell |
Sample: Colon Tissue |
PMID: 35443217 |
Sample: Serum |
DOI: 10.2147/IJN.S159789 |
|
PMID: 29695905 |
Sample: Tissue Homogenate |
PMID: 34083710 |
Sample: Saliva |
PMID: 34684685 |
Sample: Hdf Cell |
PMID: 31279280 |
Sample: Tissue Homogenate |
Sample: Hepg2 Cell |
Sample: Serum |
Sample: Liver,Kidney,Lungs |
PMID: 36235007 |
Sample: Plasma |
PMID: 35821033 |
Sample: Serum |
Sample: Serum |
Sample: Serum,Ruminal Fluid |
DOI: 10.3390/ph16020318 |
|
Sample: Lung |
Sample: Livers |
Sample: Jejunum Tissue |
DOI: 10.1039/D1FO01008E |
|
PMID: 34128029 |
Sample: Small Intestinal |
PMID: 36140930 |
Sample: Serum |
PMID: 36230124 |
Sample: Serum |
Sample: Tissue Homogenate(Brain) |
PMID: 35933747 |
Sample: Intestinal Tissue,Serum |
Sample: Achn Cell |
Sample: Heart |
PMID: 35453477 |
Sample: Blood |
PMID: 35453353 |
Sample: Serum |
Sample: Serum |
DOI: 10.1002/jcp.30283 |
|
Sample: Tissue Homogenate |
PMID: 34891114 |
Sample: Serum |
DOI: 10.1155/2021/5570331 |
|
PMID: 34257810 |
Sample: Serum |
PMID: 36037870 |
Sample: Pancreatic Tissue |
DOI: 10.1155/2022/2232365 |
|
PMID: 35898617 |
Sample: Huvecs |
DOI: 10.1155/2022/3593294 |
|
PMID: 36238648 |
Sample: Hippocampus |
Sample: Serum |
Sample: Serum,Kidney Tissue |
Sample: Kidney Tissue |
PMID: 35568865 |
Sample: Lewis Cell |
Verified Customer
F***********dSubmitted [ Sep 14 2019 ]
Verified Customer
V*****tSubmitted [ May 31 2019 ]
M*****aSubmitted [ Aug 09 2022 ]
Asked: Is the kit suitable for cell sample?
Reply
adminSubmitted [ Aug 09 2022 ]
Answered:
adminSubmitted [ Aug 09 2022 ]
Answered: No, if you want to detect cell sample, please consider the cat#E-BC-K028-M.
M*****aSubmitted [ Aug 02 2022 ]
Asked: MDA assay test, is it possible to do in ordinary lab human blood or urine?
Reply
adminSubmitted [ Aug 02 2022 ]
Answered: Sorry, it can test blood, but not urine.
j**aSubmitted [ Jul 04 2022 ]
Asked: I have used an MDA kit with code E-BC-K025-S and a CuZn-SOD/Mn-SOD kit. I want to ask where I can get the normal range of results that I get from the human serum test
Reply
adminSubmitted [ Jul 04 2022 ]
Answered: The following data are some of the results of our verification, you can also find some literatures to get the normal range ya: MDA---E-BC-K025-M---11.66 μmol/L CuZn-SOD/Mn-SOD----E-BC-K022-S---72 U/mL