MEM α was developed on the basis of MEM medium. Compared with MEM medium, NEAA, sodium pyruvate, zinc sulfate, VB12, biotin and ascorbic acid were added. It is widely used in suspension and adherent cell culture of various mammals. Mem α medium without nucleoside and deoxynucleoside is often used as medium for DG44 and other DHFR- cells. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

General Information

Product Form	Liquid
Concentration	1×
pH	7.2-7.4
D-Glucose	1000mg/L
HEPES	Negative
L-glutamine	Negative
NaHCO₃	2200mg/L
Phenol red	10mg/L
Sodium pyruvate	1 mM
Balanced salt	Earle's salt
Nucleoside	Positive
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	24 months

Notes

1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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