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Cat.No.:E-AB-F1150D
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Alternate Names | Mast/stem cell growth factor receptor Kit,Kit,SCFR,Proto-oncogene c-Kit,Tyrosine-protein kinase Kit,CD117 |
Swissprot | P10721 |
Gene ID | 3815 |
References |
1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736. |
Form | Liquid |
Clone No. | 104D2 |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | PE Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792D] |
Reactivity | Human |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
FITC Anti-Human CD117/c-Kit Antibody[104D2] | 20Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Human CD117/c-Kit Antibody[104D2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine7 Anti-Human CD117/c-Kit Antibody[104D2] | 20Tests, 100Tests, 100Tests×2 | Check More |