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PE Anti-Human CD25 Antibody[BC96]

  • Cat.No.:E-AB-F1194D

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1194D

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $30
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Antigen Details

Alternate Names Interleukin-2 receptor subunit alpha,IL2RA,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,p55
Swissprot P01589
References
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Product Details

Form Liquid
Clone No. BC96(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PE Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792D]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Human CD25 Antibody[BC96] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human CD25 Antibody[BC96] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Human CD25 Antibody[BC96] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Human CD25 Antibody[BC96] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Human CD25 Antibody[BC96] 20Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Human CD25 Antibody[BC96] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Human CD25 Antibody[BC96] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Human CD25 Antibody[BC96] 20Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Human CD25 Antibody[BC96] 20Tests, 100Tests, 100Tests×2 Check More

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. MOLECULAR PHARMACEUTICS (2022) IF: 4.939
    124I Radiolabeled Basiliximab for CD25-Targeted Immuno-PET Imaging of Activated T Cells

    DOI: 10.1021/acs.molpharmaceut.2c00330

    PMID: 35704773

    Sample: Karpas299 cell,Daudi cell

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