Click here to view cell apoptosis assay products
×

PE Anti-Human IL-4 Antibody[MP4-25D2]

Uniprot : P05112
  • Cat.No.:E-AB-F1203D

  • Applications: ICFCM
  • Isotype: Rat IgG1, κ
  • Host: Rat

To Purchase E-AB-F1203D

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $75
Qty:

Antigen Details

Alternate Names Interleukin-4,IL-4,B-cell IgG differentiation factor,B-cell growth factor 1,BSF-1,IGG1 induction factor
Swissprot P05112
References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.

Product Details

Form Liquid
Clone No. MP4-25D2(See Other Available Formats)
Host Rat
Isotype Rat IgG1, κ
Isotype Control PE Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09822D]
Reactivity Human
Application ICFCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.

Protocols & Troubleshootings

Related Protocols Staining Intracellular Antigens for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Human IL-4 Antibody[MP4-25D2] 50μg, 500μg, 1mg Check More
Purified Anti-Human IL-4 Antibody[MP4-25D2] 25μg, 100μg Check More
FITC Anti-Human IL-4 Antibody[MP4-25D2] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human IL-4 Antibody[MP4-25D2] 20Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Human IL-4 Antibody[MP4-25D2] 20Tests, 100Tests, 100Tests×2 Check More

Citations

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for 10 Test Free Trial FCM Antibody
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: