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Cat.No.:E-AB-F1227D
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Alternate Names | Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen, LCA, T200, Ly-5,Ptprc |
Swissprot | P04157 |
References |
4. Westermann J, et al. 1994. Eur. J. Immunol. 24(10):2312
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Form | Liquid |
Clone No. | OX-1(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | PE Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792D] |
Reactivity | Rat |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |