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Penicillin-Streptomycin Solution (100×)

  • Cat.No.:PB180120

To Purchase PB180120

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  • 125mL
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Background

Penicillin-streptomycin solution mixture is the most commonly used antibiotic to prevent microbial contamination in vitro. Penicillin can interfere with the synthesis of bacterial cell wall, especially for Gram-positive bacteria. Streptomycin could bind to ribosomal ribosome for 30S and inhibit the synthesis of bacterial protein. It was effective for Gram-negative bacteria and Gram-positive bacteria, but especially for Gram-negative bacteria. The combined use of penicillin and streptomycin can prevent most bacterial contamination, but penicillin solution is sensitive to temperature and pH, easy to degrade at room temperature, and needs cryopreservation, the stability of penicillin solution is the most stable when pH is 6.0 - 6.5. Streptomycin was relatively stable, and pH 5.0-7.5 was the most stable.

General Information

Product Form Liquid
Concentration 100×
pH 6.1-6.4
Dissolvent 10mM PBS (PH 6.2)
Streptomycin sulfate 10mg/mL
Penicillin G 10kU/mL
Antimicrobial spectrum Gram-positive bacteria & Gram-negative bacteria
Storage -20°C, shading light
Shipping Ice bag
Expiration date 12 months

Notes

1. This product is for research use only.
2. This product is sterilized by 0.22 μ m filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution.
4. It is not suitable for long time storage at room temperature or 4 °C.
5. Thaw in 2 -8°C and mix fully before use. Avoid repeated freezing and thawing. It is recommended to aliquot the solution and store at -20°C.
6. This product is a concentrated solution and dilute it to the desired concentration before use.

Citations

  1. Pharmaceuticals (2022) IF: 5.215
    Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells

    DOI: 10.3390/ph15091123

    PMID: 36145344

  2. BIOORGANIC CHEMISTRY (2022) IF: 5.307
    Cytotoxic steroidal saponins from the rhizomes of Paris fargesii var. petiolata

    DOI: 10.1016/j.bioorg.2022.106305

  3. Pharmaceuticals (2021) IF: 5.863
    Thymoquinone Inhibits Growth of Acute Myeloid Leukemia Cells through Reversal SHP-1 and SOCS-3 Hypermethylation: In Vitro and In Silico Evaluation

    DOI: 10.3390/ph14121287

  4. PHYTOCHEMISTRY (2022) IF: 4.004
    Cytotoxic steroidal glycosides from the rhizomes of Paris polyphylla var. yunnanensis

    DOI: 10.1016/j.phytochem.2022.113577

  5. TOXICOLOGY IN VITRO (2022) IF: 3.5
    An evaluation of a new high-sensitivity PrestoBlue assay for measuring cell viability and drug cytotoxicity using EA.hy926 endothelial cells

    DOI: 10.1016/j.tiv.2022.105407

    PMID: 35659575

  6. Asian Pacific Journal of Cancer Prevention (2021)
    Thymoquinone Induces Downregulation of BCR-ABL/JAK/STAT Pathway and Apoptosis in K562 Leukemia Cells

    DOI: 10.31557/APJCP.2021.22.12.3959

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