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Protein Carbonyl Colorimetric Assay Kit (Tissue And Serum Samples)

Cat.No.:E-BC-K117-M
Detection instrument: Microplate reader(370 nm)

Detection method: Colorimetric method

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96T

Price: $280

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Protocols for metabolism assays

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Detection principle

The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.

Performance characteristics

Sample type	serum,plasma,hydrothorax,cell culture supernatant,tissue
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	140 min
Precision	Average inter-assay CV: 8.500%Average intra-assay CV: 5.200%
Other instruments required	Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
Other reagents required	Absolute ethanol, Ethyl acetate
Storage	2-8℃
Valid period	12 months

Images

The level of carbonyl was significantly increased (P<0.05) in DMH treated groups (G3) comparing to control group (G1). Quercetin administration has inhibitory effects on protein carbonyl level in different phase (G4-G6).

S G Darband et al found that quercetin attenuated oxidative DNA damage in rats with DMH induced colon carcinogenesis, changes of markers of protein oxidation can be obtained. Protein carbonyl level of rat colon tissue was determined using protein carbonyl colorimetric assay kit (E-BC-K117-M).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Food Quality and Safety (2022) IF: 3.102

Novel Functional Food from an invasive species Polygonum cuspidatum: Safety evaluation, Chemical Composition, and Hepatoprotective Effects

DOI: 10.1093/fqsafe/fyac032
	Sample: liver

Clinical Nutrition ESPEN (2021)

Vitamin C deficiency and risk of metabolic complications among adults with chronic respiratory diseases: A case–control study

DOI: 10.1016/j.clnesp.2021.03.007
	Sample: serum

Reviews/Q&A

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Reviews
Q&A

M**********dSubmitted [ Aug 30 2022 ]

Asked: Can I use the 96-well plate instead of your microplate?

adminSubmitted [ Aug 30 2022 ]

Answered: Yes, the plate for this assay is the normal microplate without any pretreatment.

D********dSubmitted [ Aug 05 2022 ]

Asked: I found that there are E-BC-K117-M and E-BC-K117-S for protein carbonyl, what\'s the relationship or difference between them?

adminSubmitted [ Aug 05 2022 ]

Answered: These two kits are mainly the difference between the detection instruments. One is the detection of the microplate reader, and the other is the detection of the spectrophotometer.You can check our manuals.

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