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Cat.No.:E-OSEL-M0004
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Assay type | Competitive-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 5.83 pg/mL |
Sample type &Sample volume | serum and plasma; 50μL |
Specificity | This kit recognizes E3 in samples. No significant cross-reactivity or interference between E3 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of E3 concentrations in serum and plasma. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
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Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
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Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level E3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level E3 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 50.70 | 122.16 | 433.82 | 53.14 | 119.04 | 426.61 |
Standard deviation | 3.50 | 5.56 | 13.58 | 2.81 | 5.99 | 19.79 |
CV (%) | 6.90 | 4.55 | 3.13 | 5.28 | 5.03 | 4.64 |
The recovery of E3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 85-97 | 92 |
EDTA plasma(n=8) | 86-96 | 91 |
Samples were spiked with high concentrations of Mouse E3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | Estriol,E3 |
Research Area | Cell Biology, Signal transduction, Developmental biology |