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Cat.No.:E-OSEL-M0005
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Assay type | Sandwich-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 1.56-100 ng/mL |
Sensitivity | 0.51 ng/mL |
Sample type &Sample volume | serum and plasma; 50μL |
Specificity | This kit recognizes IgG in samples. No significant cross-reactivity or interference between IgG and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of IgG concentrations in serum and plasma. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IgG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IgG were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 5.07 | 8.36 | 48.61 | 5.40 | 9.15 | 48.67 |
Standard deviation | 0.29 | 0.44 | 2.38 | 0.35 | 0.46 | 1.65 |
CV (%) | 5.77 | 5.28 | 4.90 | 6.46 | 5.03 | 3.38 |
The recovery of IgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 86-101 | 93 |
EDTA plasma(n=8) | 92-107 | 99 |
Samples were spiked with high concentrations of Mouse IgG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | Immunoglobulin G,IgG |
Research Area | Immunology |
DOI: 10.2147/IJN.S338334 |
|
PMID: 35125869 |
Sample: RAW264.7 cell,splenocytes |
PMID: 30700991 |
Sample: Plasma,Tissue homogenate |
Sample: Serum |
DOI: 10.1038/srep34079 |
|
Sample: Serum |
Sample: serum |
DOI: 10.1039/D0FO01841D |
|
PMID: 32955540 |
Sample: Plasma |
PMID: 35563952 |
Sample: feces,colonic contents |
Sample: serum |
Sample: serum |
Sample: Serum |
DOI: 10.1093/jn/nxab328 |
|
Sample: blood |
PMID: 33322178 |
Sample: Serum |
Sample: serum |
PMID: 31412308 |
Sample: Serum,Tissue homogenate |
PMID: 30664205 |
Sample: Serum |