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Cat.No.:E-OSEL-M0003
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Assay type | Competitive-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 0.31-20 ng/mL |
Sensitivity | 0.14 ng/mL |
Sample type &Sample volume | serum and plasma; 50μL |
Specificity | This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum and plasma. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level T were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level T were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 1.02 | 1.87 | 8.67 | 1.00 | 2.01 | 7.85 |
Standard deviation | 0.06 | 0.11 | 0.38 | 0.06 | 0.10 | 0.43 |
CV (%) | 5.89 | 5.63 | 4.44 | 5.80 | 5.16 | 5.49 |
The recovery of T spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 93-106 | 99 |
EDTA plasma(n=8) | 84-96 | 91 |
Samples were spiked with high concentrations of Mouse T and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | Testosterone,T |
Research Area | Signal Transduction, Signal Transduction, Epigenetics and Nuclear Signaling, Developmental Biology |
PMID: 32659591 |
Sample: Serum |
Sample: serum |
PMID: 33162236 |
Sample: Serum |
PMID: 35177090 |
Sample: serum |
Sample: serum |
Sample: testis,TM3 cell |
Sample: serum |
DOI: 10.2147/IJN.S348673 |
|
PMID: 35401001 |
Sample: serum |
PMID: 35658231 |
Sample: serum |
Sample: serum |
Sample: Cell culture supernatant |
Sample: serum |
PMID: 33581723 |
Sample: Serum |
PMID: 35597951 |
Sample: serum |
DOI: 10.1042/CS20180179 |
|
Sample: Serum |
DOI: 10.1038/srep21776 |
|
Sample: serum |
Sample: testis,serum |
Sample: serum,TM3 Leydig cells |
PMID: 33213873 |
Sample: Serum |
Sample: plasma |
PMID: 34828406 |
Sample: plasma |
DOI: 10.3390/ijms20030630 |
|
PMID: 30717178 |
Sample: Tissue homogenate |
PMID: 34280466 |
Sample: Serum |
PMID: 34595713 |
Sample: serum |
DOI: 10.1038/srep43475 |
|
Sample: Serum |
PMID: 35594452 |
Sample: gonads |
DOI: 10.2147/IJN.S206561 |
|
Sample: Leydig cells |
Sample: serum |
PMID: 33360214 |
Sample: Cell culture supernatant |
PMID: 35962055 |
Sample: serum |
PMID: 34718101 |
Sample: serum |
Sample: Serum |
PMID: 30384460 |
Sample: Serum |
Sample: plasma |
Sample: Serum |
Sample: serum |
PMID: 32199951 |
Sample: Cell culture supernatant |
Sample: serum,TM3 cell |
PMID: 30195209 |
Sample: Serum |
Sample: TM3 cell |
Sample: Serum,Tissue homogenate |
PMID: 32725591 |
Sample: Serum |
DOI: 10.1155/2021/9968463 |
|
PMID: 34987599 |
Sample: serum |
Sample: serum |
Sample: Serum,Tissue homogenate |