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Cat.No.:PKSV030352
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Synonyms | S protein RBD;2019-nCoV S protein RBD;SARS-CoV-2 Spike RBD |
Species | SARS-CoV-2 |
Expression_host | HEK293 Cells |
Sequence | Arg319-Phe541(N501Y) |
Accession | YP_009724390.1 |
Mol_Mass | 26.6 kDa |
AP_Mol_Mass | 35 kDa |
Tag | C-His |
Bio_Activity | Immobilized human ACE2 protein (mFc tag)(Cat: PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His(PKSV030352), the EC50 of PKSV030352 is 3-20 ng/mL. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |