TED Focurose 6FF can be used for separation and purification with the interactions between chelated metal ions and some amino acids (mainly include histidine, cysteine, tryptophan) on the side chain of protein, and it is suitable for separation and purification of His-tagged protein and biological molecules which interact with metal ions. The strong binding metal ions can be directly used for His-tagged protein expressed by eukaryotic expression system, and it have resistance for higher concentration of reductant and chelating agent. In addition, sample pretreatment is unnecessary. The cleaning and reviving of media are simple, which can be washed directly without nickel removal.

Product Features

1. Quick and easy (one-step purification).
2. Tolerate to higher concentration of reductant and chelating agent. His-tagged protein expressed by eukaryotic expression system can be loading without pretreatment, which can protect the protein activity to the utmost.
3. There is no need to remove nickel removal can wash with NaOH directly, which greatly shorten the cleaning period.
4. Multiple selection. Various metal ions (e.g. Cu2+, Zn2+, Fe2+, Co2+, Ca2+, etc.) can be used.

Product Datasheet

Matrix	Highly cross-linked 6% agarose
Particle size range	45-165 µm
Average particle size	90 µm
Binding capacity	20 mg(His-tagged protein)/mL(media)
Media type	Affinity Chromatography Medium
pH stability	3-12(working), 2-14(washing)
Chemical stability	0.01MHCl, 0.01M NaOH(1 week)20 mM EDTA, 10 mM DTT, 1M NaOH, 8M Urea, 6M Guanidine hydrochloride(24 hours)100 mM EDTA, 0.5M Iminazole(2 hours)30% Isopropyl alcohol(20 minutes)
Maximum flow rate	600 cm/h
Pressure	≤ 0.3 Mpa
Storage buffer	20% Ethanol
Storage temperature	4~30℃(4~8℃ is preferred)

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Cat. No	E-CM-AF26
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