Form	Liquid
Clone No.	3G8
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	PE Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792D]
Reactivity	Human
Application	FCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD16 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD16 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD16 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD16 appears to be expressed later in the immune response than CD86. CD16 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Human CD16 Antibody[3G8]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD16 Antibody[3G8]	20Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Human CD16 Antibody[3G8]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD16 Antibody[3G8]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD16 Antibody[3G8]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD16 Antibody[3G8]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD16 Antibody[3G8]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD16 Antibody[3G8]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD16 Antibody[3G8]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Human CD16 Antibody[3G8]	20Tests, 100Tests, 100Tests×2	Check More

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