Introduction
Red Blood Cell Lysis Buffer
(RBC Lysis), also known as ACK Lysis Buffer, is a kind of solution used to lyse
and remove enucleated red blood cells from blood or tissue samples of human or
mouse.
This product is sterile.
After treating with this buffer, the single cell suspensions can be used for
subsequent primary culture, cell fusion, extraction of nucleic acid or protein,
and various routine analysis and detection.
Instructions
10×ACK Lysis Buffer is concentrated, dilute with ddH2O to 1× working
solution before use.
For example: take 1 mL 10× ACK Lysis Buffer [E-CK-A105], add to 9 mL ddH2O.The
mixture is 1× ACK Lysis Working Buffer.
Components
|
Cat.
|
Products
|
100 mL
|
200 mL
|
500 mL
|
Storage
|
|
E-CK-A105
|
10× ACK
Lysis Buffer
|
100 mL
|
200 mL
|
500 mL
|
2~8°C
|
|
Manual
|
One Copy
|
Experimental Procedure
For Mouse Spleen or Bone Marrow
Samples
1. Centrifuge single
cell suspension of the sample at 300 g for 5 min, discard the supernatant.
2. Add 2 mL of 1
×ACK Lysis Working Buffer to the sample to resuspend the cells, and incubate at
RT for 2~3 min (Incubation time should be adjusted accordingly to the actual
situation).
3. Add 10 mL PBS
to stop the lysis reaction.
4. Centrifuge at 300 g for 5
min, discard the supernatant.
5. Resuspend the
cells with PBS or similar physiological buffer for subsequent experiments.
For Human Peripheral Blood
Samples
1. Add 100 μL
fresh blood into a centrifuge tube, add 2 mL of 1 ×ACK Lysis Working Buffer and
mix thoroughly.
2. Put the
centrifuge tube at
4°C for 10 min, until the fresh blood becomes transparent.
3. Centrifuge at 300 g for 5 min, discard the supernatant.
Use a pipette to remove the residual supernatant carefully.
4. Add 2 mL PBS
to the tube and repeat step 3.
5. Resuspend the
cells with PBS or similar physiological buffer for subsequent experiments.
Storage
Store
at 2~8°C. Avoid of freezing. Valid for 12 months.
Cautions
1.
This product is sterile. Please open and use it in laminar
flow bench..
2. This reagent is for research
use only.
3. For your safety and health,
please wear the lab coat and disposable gloves before the experiments.
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