Click here to view cell apoptosis assay products
×

BCA Protein Colorimetric Assay Kit

  • Cat.No.:E-BC-K318-M

  • Detection instrument: Microplate reader(540-590 nm, optimum wavelength: 562 nm)

To Purchase E-BC-K318-M

Size:
  • 500Assays
  • 96T
Price: $150
Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

 Add to cart Bulk request

For research use only. Order now, ship in 3 days

★: Microplate is not provided in metabolism assay kits of over 500 assays.

Detection principle

Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.

Performance characteristics

Synonyms TP
Sample type Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity 0.0165 mg/mL
Detection range 0.0165-1 mg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 40 min
Precision Average inter-assay CV: 4.500%Average intra-assay CV: 2.200%
Other instruments required Vortex mixer, Micropipettor, Incubator
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage RT
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.0165-1 mg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Mouse brain tissue homogenization

8-12

10% Mouse kidney tissue homogenization

8-12

Human serum

100-200

10% Rat liver tissue homogenization

15-20

10% Mouse heart tissue homogenization

8-12

Rat serum

100-200

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. Frontiers in Pharmacology (2022) IF: 5.811
    Tilianin Reduces Apoptosis via the ERK/EGR1/BCL2L1 Pathway in Ischemia/Reperfusion-Induced Acute Kidney Injury Mice.

    DOI: 10.3389/fphar.2022.862584

    Sample: Kidney Tissue
  2. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo

    DOI: 10.3390/ijms232416134

    Sample: Lung Tissue,Thp-1 Cells
  3. Brazilian Journal of Cardiovascular Surgery (2020) IF: 1.053
    Effect of Shenfu Injection on Isolated Empty Beating Hearts from Miniature Pigs

    DOI: 10.21470/1678-9741-2019-0264

    Sample: Tissue Homogenate
  4. NEUROREPORT (2021) IF: 1.837
    Protective effects of melatonin on the white matter damage of neonatal rats by regulating NLRP3 inflammasome activity

    DOI: 10.1097/WNR.0000000000001642

    PMID: 33994520

    Sample: Periventricular
  5. NEUROSCIENCE LETTERS (2018) IF: 2.159
    Regular aerobic exercise correlates with reduced anxiety and incresed levels of irisin in brain and white adipose tissue

    DOI: 10.1016/j.neulet.2018.04.023

    Sample: Serum,Plasma,Whole Blood,Platelet,Tissue Homogenat
  6. ARCHIVES OF GYNECOLOGY AND OBSTETRICS (2022) IF: 2.344
    Effect of Neiyi Prescription of QIU on autophagy and angiogenic ability of endometriosis via the PPARγ/NF-κB signaling pathway

    DOI: 10.1007/s00404-022-06537-w

    PMID: 35366690

    Sample: Ectopic Endometria
  7. ENDOCRINE JOURNAL (2022) IF: 2.349
    Human umbilical cord mesenchymal stem cells alleviate the imbalance of CD4+ T cells via protein tyrosine phosphatase non-receptor type 2/signal transducer and activator of transcription 3 signaling in ameliorating experimental autoimmune thyroiditis in ra

    DOI: 10.1507/endocrj.EJ20-0695

    Sample: Cd4+ T Cell
  8. BIOLOGICAL TRACE ELEMENT RESEARCH (2018) IF: 2.361
    Timeline (Bioavailability) of Magnesium Compounds in Hours: Which Magnesium Compound Works Best?

    DOI: 10.1007/s12011-018-1351-9

    Sample: Brain Tissues,Plasma,Muscle Tissues
  9. Experimental and Therapeutic Medicine (2022) IF: 2.447
    Salvianolic acid A promotes mitochondrial biogenesis and function via regulating the AMPK/PGC‑1α signaling pathway in HUVECs

    DOI: 10.3892/etm.2022.11412

    Sample: Primary Huvecs
  10. ANDROLOGIA (2021) IF: 2.775
    Runjing Decoction alleviated cyclophosphamide-induced oligoasthenospermia rats by inhibiting cell apoptosis via RXFP1/AKT/FOXO1 pathway

    DOI: 10.1111/and.14216

    PMID: 34396564

    Sample: Testis Tissue
  11. Journal of Pain Research (2023) IF: 2.832
    The Role of IL-6 and TMEM100 in Lumbar Discogenic Pain and the Mechanism of the Glycine-Serine-Threonine Metabolic Axis: A Metabolomic and Molecular Biology Study

    DOI: 10.2147/JPR.S400871

    Sample: Dorsal Root Ganglion (Drg) Tissue,Intervertebral
  12. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2019) IF: 2.914
    Nicotine increased VEGF and MMP2 levels in the rat eye and kidney

    DOI: 10.1007/s11356-019-06460-9

  13. JOURNAL OF BIOENERGETICS AND BIOMEMBRANES (2022) IF: 2.945
    CircCDC6 restrains tumor growth and glycolysis energy metabolism in colorectal cancer via regulating miR-3187-3p and downstream PRKAA2

    DOI: 10.1007/s10863-022-09938-3

    PMID: 35438362

    Sample: Sw480 Cell,Hct116 Cell
  14. GENE (2020) IF: 2.984
    Chitosan-stabilized selenium nanoparticles alleviate cardio-hepatic damage in type 2 diabetes mellitus model via regulation of caspase, Bax/Bcl-2, and Fas/FasL-pathway

    DOI: 10.1016/j.gene.2020.145288

    PMID: 33181259

    Sample: Serum
  15. Journal of Food Measurement and Characterization (2022) IF: 3.006
    Isolation and structural characterization of antioxidant peptides from horse bone marrow protein hydrolysates

    DOI: 10.1007/s11694-022-01638-1

    Sample: Bone Marrow
  16. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    A relationship between MAPK/ERK pathway expression and neuronal apoptosis in rats with white matter lesions

    DOI: 10.26355/eurrev_202004_21023

    PMID: 32373979

    Sample: Tissue Homogenate
  17. Journal of Integrative Medicine-JIM (2022) IF: 3.034
    Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway

    DOI: 10.1016/j.joim.2022.01.007

    PMID: 35153135

    Sample: Ovarian Tissue
  18. Frontiers in Bioscience-Landmark (2022) IF: 3.115
    SIRT3 Enhances the Protective Role of Propofol in Postoperative Cognitive Dysfunction via Activating Autophagy Mediated by AMPK/mTOR Pathway

    DOI: 10.31083/j.fbl2711303

    Sample: Hippocampal Tissue,Primary Neurons
  19. EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
    The antidepressant effects of hesperidin on chronic unpredictable mild stress-induced mice

    DOI: 10.1016/j.ejphar.2019.03.035

    PMID: 30928632

    Sample: Cell Culture Supernatant,Tissue Homogenate
  20. Frontiers in Physiology (2019) IF: 3.201
    Dietary Bile Acids Enhance Growth, and Alleviate Hepatic Fibrosis Induced by a High Starch Diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 Pathway in Micropterus salmoides

    DOI: 10.3389/fphys.2019.01430

    PMID: 31824338

    Sample: Plasma
  21. PLoS One (2022) IF: 3.24
    Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats

    DOI: 10.1371/journal.pone.0266898

    PMID: 35421180

    Sample: Brain Tissue
  22. MOLECULES (2020) IF: 3.267
    The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury

    DOI: 10.3390/molecules25204612

    PMID: 33050458

    Sample: Cell Lysate
  23. Bioengineered (2022) IF: 3.269
    Tanshinone IIA alleviates cardiac hypertrophy through m6A modification of galectin-3

    DOI: 10.1080/21655979.2022.2031388

    PMID: 35191812

  24. Bioengineered (2021) IF: 3.269
    Downregulation of Circular RNA PSEN1 ameliorates ferroptosis of the high glucose treated retinal pigment epithelial cells via miR-200b-3p/cofilin-2 axis

    DOI: 10.1080/21655979.2021.2010369

    PMID: 34903141

    Sample: Arpe19 Cell
  25. Diabetology & Metabolic Syndrome (2022) IF: 3.32
    LncRNA HOTAIR facilitates high glucose-induced mesangial cell proliferation, fibrosis and oxidative stress in diabetic nephropathy via regulating miR-147a/WNT2B axis

    DOI: 10.1186/s13098-022-00802-3

    PMID: 35193668

  26. JOURNAL OF PHARMACOLOGICAL SCIENCES (2021) IF: 3.337
    Sesamin promotes apoptosis and pyroptosis via autophagy to enhance antitumour effects on murine T-cell lymphoma

    DOI: 10.1016/j.jphs.2021.08.001

    PMID: 34507635

    Sample: El4 Cell
  27. POULTRY SCIENCE (2021) IF: 3.352
    Adiponectin modulates steroid hormone secretion, granulosa cell proliferation and apoptosis via binding its receptors during hens’ high laying period

    DOI: 10.1016/j.psj.2021.101197

    Sample: Tissue Homogenate
  28. Brain and Behavior (2022) IF: 3.405
    The protective effects of nesfatin-1 in neurological dysfunction after spinal cord injury by inhibiting neuroinflammation

    DOI: 10.1002/brb3.2778

    PMID: 36271663

  29. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS (2023) IF: 3.405
    Copper-deposited diatom-biosilica enhanced osteogenic potential in periodontal ligament stem cells and rat cranium

    DOI: 10.1002/jbm.b.35232

    Sample: Hpdlscs
  30. Frontiers in Veterinary Science (2022) IF: 3.412
    Rutin Supplementation Reduces Oxidative Stress, Inflammation and Apoptosis of Mammary Gland in Sheep During the Transition Period

    DOI: 10.3389/fvets.2022.907299

    Sample: Mammary Gland Tissue
  31. Canadian Journal of Gastroenterology and Hepatology (2022) IF: 3.522
    LncRNA PVT1 Promotes Cell Proliferation, Invasion, and Migration and Inhibits Cell Apoptosis by Phosphorylating YAP

    DOI: 10.1155/2022/5332129

    PMID: 35664988

    Sample: Mkn-45 Cell
  32. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2021) IF: 3.575
    Downregulation of circular RNA circDOCK7 identified from diabetic rats after sleeve gastrectomy contributes to hepatocyte apoptosis through regulating miR-139–3p and MCM3

    DOI: 10.1016/j.bbrc.2021.02.069

    Sample: Cell Lysate(Brl Cell Lines (Normal Rat Hepatocytes
  33. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Network pharmacology, molecular docking and in vivo and in vitro experiments to explore the molecular mechanism of licorice green tea beverage to scavenge oxygen free radicals

    DOI: 10.1111/jfbc.14315

    PMID: 35855584

    Sample: Hacat Cell
  34. Frontiers in Neuroscience (2020) IF: 3.707
    Potent Natural Antioxidant Carveol Attenuates MCAO-Stress Induced Oxidative, Neurodegeneration by Regulating the Nrf-2 Pathway

    DOI: 10.3389/fnins.2020.00659

    Sample: Tissue Homogenate
  35. BRAIN RESEARCH BULLETIN (2022) IF: 3.715
    Chuanzhitongluo regulates microglia polarization and inflammatory response in acute ischemic stroke

    DOI: 10.1016/j.brainresbull.2022.09.015

    PMID: 36152772

    Sample: Brain Tissue
  36. Heliyon (2023) IF: 3.776
    Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions

    DOI: 10.1016/j.heliyon.2023.e12958

    Sample: Htr-8/Svneo Cell
  37. MICROBIAL PATHOGENESIS (2022) IF: 3.848
    Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro

    DOI: 10.1016/j.micpath.2022.105964

    Sample: Bone Marrow-Derived Macrophages (Bmms)
  38. EXPERIMENTAL CELL RESEARCH (2022) IF: 3.905
    p38MAPK silencing attenuates scar proliferation after cleft palate repair surgery in rats via MRTF-A/SRF pathway

    DOI: 10.1016/j.yexcr.2022.113248

    PMID: 35690133

    Sample: Fibroblast
  39. Annals of Translational Medicine (2022) IF: 3.932
    SIRT3-mediated mitochondrial autophagy in refeeding syndrome-related myocardial injury in sepsis rats

    DOI: 10.21037/atm-22-222

    PMID: 35280405

    Sample: Cardioid
  40. Annals of Translational Medicine (2022) IF: 3.932
    Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

    DOI: 10.21037/atm-22-483

    PMID: 35280364

    Sample: Myocardium
  41. Biomolecules (2020) IF: 4.082
    Post-Treatment of Synthetic Polyphenolic 1,3,4 Oxadiazole Compound A3, Attenuated Ischemic Stroke-Induced Neuroinflammation and Neurodegeneration

    DOI: 10.3390/biom10060816

    PMID: 32466476

    Sample: Tissue Homogenate
  42. ENVIRONMENTAL TOXICOLOGY (2021) IF: 4.119
    Compound essential oils relieve oxidative stress caused by PM2.5 exposure by inhibiting autophagy through the AMPK/mTOR pathway

    DOI: 10.1002/tox.23297

    Sample: Cell Culture Supernatant
  43. BIOLOGY OF REPRODUCTION (2023) IF: 4.161
    Extracellular vesicles contribute to EMT in Adenomyosis by inducing macrophage polarization

    DOI: 10.1093/biolre/ioad015

    Sample: Primary Endometrial Cell
  44. Human Cell (2022) IF: 4.174
    USP36 promotes tumor growth of non-small cell lung cancer via increasing KHK-A expression by regulating c-MYC–hnRNPH1/H2 axis

    DOI: 10.1007/s13577-022-00677-6

    Sample: A549 Cell,Pc-9 Cell,Nci-H292 Cell,Nci-1975 Cell,Be
  45. GENOMICS (2022) IF: 4.31
    CircEML1 facilitates the steroid synthesis in follicular granulosa cells of chicken through sponging gga-miR-449a to release IGF2BP3 expression

    DOI: 10.1016/j.ygeno.2022.110540

    Sample: Granulosa Cells (Gcs)
  46. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Revealing the mechanism of “Huai Hua San” in the treatment of ulcerative colitis based on network pharmacology and experimental study

    DOI: 10.1016/j.jep.2021.114321

  47. ACS Chemical Neuroscience (2021) IF: 4.418
    Synthesis and Biological Evaluation of Benzimidazole Derivatives as Potential Neuroprotective Agents in an Ethanol-Induced Rodent Model

    DOI: 10.1021/acschemneuro.0c00659

    PMID: 33430586

    Sample: Tissue Homogenate(Brain)
  48. JOURNAL OF PERIODONTAL RESEARCH (2022) IF: 4.419
    Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells

    DOI: 10.1111/jre.12973

    PMID: 35037251

    Sample: Periodontal Ligament Stem Cell (Pdlscs)
  49. Frontiers in Physiology (2021) IF: 4.566
    Sleeve Gastrectomy Ameliorates Diabetes-Induced Cardiac Hypertrophy Correlates With the MAPK Signaling Pathway

    DOI: 10.3389/fphys.2021.785799

    PMID: 34858216

    Sample: Heart
  50. Frontiers in Neuroscience (2022) IF: 4.677
    Neuroprotective Effect of Otostegia limbata Against PTZ-Induced Mice Model of Epilepsy by Attenuated Expression of p-NFκB and TNF-α

    DOI: 10.3389/fnins.2022.779681

    PMID: 35392411

    Sample: Cortex,Hippocampus
  51. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2022) IF: 4.925
    Gallic Acid Ameliorates Aspergillus Fumigatus Keratitis Through Reducing Fungal Load and Suppressing the Inflammatory Response

    DOI: 10.1167/iovs.63.12.12

  52. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Quercetin ameliorates Aspergillus fumigatus keratitis by inhibiting fungal growth, toll-like receptors and inflammatory cytokines

    DOI: 10.1016/j.intimp.2021.107435

    Sample: Tissue Homogenate(Corneas)
  53. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Pravastatin attenuates sepsis-induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of Cav-1/eNOS pathway

    DOI: 10.1016/j.intimp.2021.108077

    PMID: 34464887

    Sample: Balf
  54. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Isoliquiritigenin alleviates P. gingivalis-LPS/ATP-induced pyroptosis by inhibiting NF-κB/ NLRP3/GSDMD signals in human gingival fibroblasts

    DOI: 10.1016/j.intimp.2021.108338

    PMID: 34794890

  55. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    High-density lipoprotein reduces microglia activation and protects against experimental autoimmune encephalomyelitis in mice

    DOI: 10.1016/j.intimp.2022.108566

    PMID: 35101849

    Sample: Spinal Cord,Bv2 Microglial Cell
  56. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Effect of gingival mesenchymal stem cell-derived exosomes on inflammatory macrophages in a high-lipid microenvironment

    DOI: 10.1016/j.intimp.2021.107455

    PMID: 33582592

    Sample: Cell Culture Supernatant
  57. LWT-FOOD SCIENCE AND TECHNOLOGY (2021) IF: 4.952
    Bio-electrochemical impedance analysis of frozen Japanese pear tissues: And the relationships among the physical properties, total polyphenol content, and oxidase activity

    DOI: 10.1016/j.lwt.2021.112499

    Sample: Fruit
  58. EUROPEAN JOURNAL OF MEDICAL RESEARCH (2022) IF: 4.981
    Inhibition of p38 mitogen-activated protein kinases may attenuate scar proliferation after cleft lip surgery in rabbits via Smads signaling pathway

    DOI: 10.1186/s40001-022-00757-1

    PMID: 35858881

    Sample: Fibroblast
  59. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2022) IF: 5
    m6A methyltransferase METTL3 participated in sympathetic neural remodeling post-MI via the TRAF6/NF-κB pathway and ROS production

    DOI: 10.1016/j.yjmcc.2022.06.004

    PMID: 35717715

    Sample: Cardiac Tissue
  60. EUROPEAN JOURNAL OF PHARMACOLOGY (2023) IF: 5.195
    Pentoxifylline treats Aspergillus fumigatus keratitis by reducing fungal burden and suppressing corneal inflammation

    DOI: 10.1016/j.ejphar.2023.175607

    Sample: Corneas,Raw 264.7 Cell
  61. Frontiers in Chemistry (2022) IF: 5.221
    Exosomes Derived From Human Gingival Mesenchymal Stem Cells Attenuate the Inflammatory Response in Periodontal Ligament Stem Cells

    DOI: 10.3389/fchem.2022.863364

    PMID: 35464198

    Sample: Gmscs
  62. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022) IF: 5.31
    New insights into the central sympathetic hyperactivity post-myocardial infarction: Roles of METTL3-mediated m6A methylation

    DOI: 10.1111/jcmm.17183

    Sample: Brain Tissue
  63. Food Bioscience (2022) IF: 5.318
    Phytosterol of lotus seed core powder alleviates hypercholesterolemia by regulating gut microbiota in high-cholesterol diet-induced C57BL/6J mice

    DOI: 10.1016/j.fbio.2022.102279

    Sample: Serum,Liver Tissue,Fecal Lipids
  64. Food & Function (2022) IF: 5.396
    Screening and identification of a novel antidiabetic peptide from collagen hydrolysates of Chinese giant salamander skin: network pharmacology, inhibition kinetics and protection of IR-HepG2 cells

    DOI: 10.1039/D1FO03527D

    PMID: 35260876

    Sample: Hepg2 Cell
  65. JOURNAL OF DERMATOLOGICAL SCIENCE (2022) IF: 5.408
    Sestrin2 Contributes to BRAF Inhibitor Resistance Via Reducing Redox Vulnerability of Melanoma Cells

    DOI: 10.1016/j.jdermsci.2022.12.007

    Sample: 451Lu Cells,Uacc62 Cells
  66. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2018) IF: 5.5
    microRNA-15 Activates NF-κB Pathway via Down Regulating Expression of Adenosine A2 Receptor in Ulcerative Colitis

    DOI: 10.1159/000495718

    Sample: Colonic Tissues,Ht-29 Cells
  67. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Mechanism of antifungal activity and therapeutic action of β-ionone on Aspergillus fumigatus keratitis via suppressing LOX1 and JNK/p38 MAPK activation

    DOI: 10.1016/j.intimp.2022.108992

    PMID: 35810488

    Sample: Corneas
  68. INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
    Dimethyl fumarate ameliorates fungal keratitis by limiting fungal growth and inhibiting pyroptosis

    DOI: 10.1016/j.intimp.2023.109721

    Sample: Corneal Tissue,Human Corneal Epithelial Cells (Hce
  69. Stem Cell Reviews and Reports (2022) IF: 5.739
    Adipose Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing Through the WNT/β-catenin Signaling Pathway in Dermal Fibroblasts

    DOI: 10.1007/s12015-022-10378-0

    PMID: 35471485

    Sample: Admsc-Exos
  70. Frontiers in Plant Science (2022) IF: 5.754
    MC03g0810, an Important Candidate Gene Controlling Black Seed Coat Color in Bitter Gourd (Momordica spp.).

    DOI: 10.3389/fpls.2022.875631

    Sample: Seed Coat
  71. Pharmaceuticals (2021) IF: 5.863
    Fucoidan Protects against Acute Sulfoxaflor-Induced Hematological/Biochemical Alterations and Oxidative Stress in Male Mice

    DOI: 10.3390/ph15010016

    Sample: Serum
  72. Frontiers in Pharmacology (2022) IF: 5.988
    Ferredoxin 1 is a cuproptosis-key gene responsible for tumor immunity and drug sensitivity: A pan-cancer analysis.

    DOI: 10.3389/fphar.2022.938134

    Sample: Blca Cell,Ccrcc Cell,Pca Cell
  73. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats

    DOI: 10.3390/ijms231810920

    PMID: 36142831

    Sample: Muscle
  74. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 6.291
    Low trifloxystrobin-tebuconazole concentrations induce cardiac and developmental toxicity in zebrafish by regulating notch mediated‐oxidative stress generation

    DOI: 10.1016/j.ecoenv.2022.113752

    Sample: Embryo
  75. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    Carveol Attenuates Seizure Severity and Neuroinflammation in Pentylenetetrazole-Kindled Epileptic Rats by Regulating the Nrf2 Signaling Pathway

    DOI: 10.1155/2021/9966663

    PMID: 34422216

    Sample: Brain
  76. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2023) IF: 6.633
    A prospective diagnostic and prognostic biomarker for hepatocellular carcinoma that functions in glucose metabolism regulation: Solute carrier family 37 member 3

    DOI: 10.1016/j.bbadis.2023.166661

    Sample: Hl7702 Cell,Hepg2 Cell,Huh-7 Cell
  77. BRAIN BEHAVIOR AND IMMUNITY (2021) IF: 7.217
    Acute neuroinflammation increases excitability of prefrontal parvalbumin interneurons and their functional recruitment during novel object recognition

    DOI: 10.1016/j.bbi.2021.08.216

    PMID: 34403738

    Sample: Medial Prefrontal Cortex (Mpfc)
  78. Materials Today Bio (2021) IF: 7.348
    Cascade targeting codelivery of ingenol-3-angelate and doxorubicin for enhancing cancer chemoimmunotherapy through synergistic effects in prostate cancer

    DOI: 10.1016/j.mtbio.2021.100189

    Sample: Rm-1 Cell,Lncap Pca Cell
  79. JOURNAL OF MEDICINAL CHEMISTRY (2022) IF: 7.446
    MPLA-Adjuvanted Liposomes Encapsulating S-Trimer or RBD or S1, but Not S-ECD, Elicit Robust Neutralization Against SARS-CoV-2 and Variants of Concern

    DOI: 10.1021/acs.jmedchem.1c02025

    PMID: 35108485

    Sample: Liposome
  80. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
    BMSC exosome-enriched acellular fish scale scaffolds promote bone regeneration

    DOI: 10.1186/s12951-022-01646-9

    PMID: 36224596

    Sample: Bmscs
  81. SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753
    Triclocarban and triclosan exacerbate high-fat diet-induced hepatic lipid accumulation at environmental related levels: The potential roles of estrogen-related receptors pathways

    DOI: 10.1016/j.scitotenv.2022.160079

  82. CHEMICAL ENGINEERING JOURNAL (2023) IF: 16.744
    Right once for all: Zinc-modulated highly stable iron-based ROS generator under physiological conditions for promoting bacteria-infected wound healing

    DOI: 10.1016/j.cej.2023.141837

  83. IMMUNITY (2023) IF: 43.474
    Pregnancy-induced changes to the gut microbiota drive macrophage pyroptosis and exacerbate septic inflammation

    DOI: 10.1016/j.immuni.2023.01.015

    PMID: 36792573

    Sample: Bm-Derived Macrophages (Bmdms)
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

T****************nSubmitted [ Jan 10 2024 ]

Asked: can this kit use to measure protein in zebrafish tissue samples,tq

Reply

A

adminSubmitted [ Jan 10 2024 ]

Answered: This kit can be used to measure tissue samples regardless of species. Our laboratory has not verified the zebrafish tissue samples, but theoretically, if the protein content of the samples is higher than the sensitivity of the kit, it can be detected.

Q

aSubmitted [ Dec 08 2023 ]

Asked: can this be used to assess stool protein level

Reply

Q

M***********dSubmitted [ Jun 11 2023 ]

Asked: Can this kit be used for bronchoalveolar lavage samples? And what should be the dilution factor of mouse lung tissue samples

Reply

A

adminSubmitted [ Jun 11 2023 ]

Answered: Hello Muntaha javid,thanks for your support.Our kit is suitable for bronchoalveolar lavage samples.Lung tissue sample processing: 10% sample homogenate diluted 10 times.

Q

M*****aSubmitted [ Aug 01 2022 ]

Asked: E-BC-K318-M is ok for use with Human salive samples. If so, can you please send me salive samples method ?

Reply

A

adminSubmitted [ Aug 01 2022 ]

Answered: Saliva: gargling with clear water, after 30 min saliva was collected and centrifuged at 10000 g at 4℃ for 5 min. Then take the supernatant for detection. If not detected on the same day, the saliva can be stored at-80℃ for a month.

Q

M*****aSubmitted [ Aug 01 2022 ]

Asked: How long is the shelf life of this kit

Reply

A

adminSubmitted [ Aug 01 2022 ]

Answered:  The shelf life of this kit is 12 months.

... Show All Show Less

People Also Bought

Apply for FREE TRIAL Fluorometric Assay kit
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: