Malate and NAD+ form oxoacetate and NADH under the action of malate dehydrogenase, then acetyl-coa and oxoacetate form coenzyme A and citrate under the action of citrate synthetase. The content of acetyl-coA can be calculated by measuring the change of absorbance value at 340 nm

Performance characteristics

Synonyms	Acetyl-CoA
Sample type	animal tissue
Sensitivity	150 nmol/mL
Detection range	150-500 nmol/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	15 min
Precision	Average inter-assay CV: 5.300%Average intra-assay CV: 4%
Storage	-20℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

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Cat. No	E-BC-K652-M
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