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Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

Cat.No.:E-BC-F038
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

Detection method: Fluorescence method

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Detection principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Performance characteristics

Synonyms	ALT
Sample type	Serum, plasma, animal tissue
Sensitivity	0.01 U/L
Detection range	0.01-0.83 U/L
Detection method	Fluorescence method
Assay type	Enzyme Activity
Assay time	75 min
Precision	Average inter-assay CV: 9.800%Average intra-assay CV: 2.300%
Other instruments required	Pipettor, Vortex mixer, Centrifuge
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-0.83 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	10-15
Dog serum	5-10
Rat serum	10-15
10% Mouse heart tissue homogenate	100-120
10% Rat spleen tissue homogenate	10-15
10% Rat liver tissue homogenate	300-500
10% Rat kidney tissue homogenate	100-120
10% Rat lung tissue homogenate	100-120

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

ACS Applied Materials & Interfaces (2019) IF: 8.456

Multifunctional Graphene-Oxide-Reinforced Dissolvable Polymeric Microneedles for Transdermal Drug Delivery

DOI: 10.1021/acsami.9b19518
	Sample: blood

CELL PROLIFERATION (2022) IF: 8.7550

Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal

DOI: 10.1111/cpr.13332
	Sample: serum

Acta Biomaterialia (2021) IF: 8.9469

A solid lipid coated calcium peroxide nanocarrier enables combined cancer chemo/chemodynamic therapy with O2/H2O2 self-sufficiency

DOI: 10.1016/j.actbio.2020.12.036
	Sample: Serum,Plasma

FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025

Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1

DOI: 10.1016/j.fct.2021.112124
PMID: 33727180	Sample: Serum

Metabolites (2022) IF: 5.581

Effect of Linoleic Acid on Cholesterol Levels in a High-Fat Diet-Induced Hypercholesterolemia Rat Model

DOI: 10.3390/metabo13010053
	Sample: serum

Scientific Reports (2022) IF: 4.996

Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

DOI: 10.1038/s41598-022-21816-x
	Sample: serum

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