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Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

  • Cat.No.:E-BC-F038

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

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  • 96T
  • 48T
Price: $400
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Detection principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Performance characteristics

Synonyms ALT
Sample type Serum, plasma, animal tissue
Sensitivity 0.01 U/L
Detection range 0.01-0.83 U/L
Detection method Fluorescence method
Assay type Enzyme Activity
Assay time 75 min
Precision Average inter-assay CV: 9.800%Average intra-assay CV: 2.300%
Other instruments required Pipettor, Vortex mixer, Centrifuge
Storage -20℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-0.83 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

10-15

Dog serum

5-10

Rat serum

10-15

10% Mouse heart tissue homogenate

100-120

10% Rat spleen tissue homogenate

10-15

10% Rat liver tissue homogenate

300-500

10% Rat kidney tissue homogenate

100-120

10% Rat lung tissue homogenate

100-120

Note: The diluent is reagent 1.

Citations

  1. FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
    Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1

    DOI: 10.1016/j.fct.2021.112124

    PMID: 33727180

    Sample: Serum
  2. Scientific Reports (2022) IF: 4.996
    Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

    DOI: 10.1038/s41598-022-21816-x

    Sample: Serum
  3. Metabolites (2022) IF: 5.581
    Effect of Linoleic Acid on Cholesterol Levels in a High-Fat Diet-Induced Hypercholesterolemia Rat Model

    DOI: 10.3390/metabo13010053

    Sample: Serum
  4. ACS Applied Materials & Interfaces (2019) IF: 8.456
    Multifunctional Graphene-Oxide-Reinforced Dissolvable Polymeric Microneedles for Transdermal Drug Delivery

    DOI: 10.1021/acsami.9b19518

    Sample: Blood
  5. CELL PROLIFERATION (2022) IF: 8.755
    Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal

    DOI: 10.1111/cpr.13332

    Sample: Serum
  6. Acta Biomaterialia (2021) IF: 8.947
    A solid lipid coated calcium peroxide nanocarrier enables combined cancer chemo/chemodynamic therapy with O2/H2O2 self-sufficiency

    DOI: 10.1016/j.actbio.2020.12.036

    Sample: Serum,Plasma
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