Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

Performance characteristics

Synonyms	ALP
Sample type	Serum,plasma,tissue,cells
Sensitivity	0.13 King unit/100 mL
Detection range	0.13-50 King unit/100 mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 8.500%Average intra-assay CV: 5.100%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.13-50 King unit/100 mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	1
Human urine	1
Rat serum	1
Cells culture supernatant	1
10% Mouse kidney tissue homogenate	30-50
0% Mouse liver tissue homogenate	1
10% Mouse brain tissue homogenate	1
HepG2 cells	1

Note: The diluent is normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

BIOCHEMICAL PHARMACOLOGY (2022) IF: 6.1

Pterostilbene-isothiocyanate impedes RANK/TRAF6 interaction to inhibit osteoclastogenesis, promoting osteogenesis in vitro and alleviating glucocorticoid induced osteoporosis in rats

DOI: 10.1016/j.bcp.2022.115284
PMID: 36209841	Sample: Serum,Mc3T3-E1 Cells,Hfob 1.19 Cells

Frontiers in Pharmacology (2022) IF: 5.811

Uncovering the Pharmacological Mechanisms of Gexia-Zhuyu Formula (GXZY) in Treating Liver Cirrhosis by an Integrative Pharmacology Strategy.

DOI: 10.3389/fphar.2022.793888
	Sample: Serum

Toxicology Reports (2022)

Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats

DOI: 10.1016/j.toxrep.2022.06.005
	Sample: Plasma

Toxicology Reports (2017)

Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

DOI: 10.1016/j.toxrep.2017.12.002
	Sample: Plasma

BMC MUSCULOSKELETAL DISORDERS (2022) IF: 2.562

miR-215-5p regulates osteoporosis development and osteogenic differentiation by targeting XIAP

DOI: 10.1186/s12891-022-05731-w
PMID: 35978328	Sample: Hbmsc

Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65

San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

DOI: 10.1155/2022/7832540
	Sample: Hepatic Tissue,Serum

Molecular Medicine Reports (2020) IF: 2.952

Mechanisms by which electroacupuncture‑mediated histone acetylation mitigates bone loss in rats with ovariectomy‑induced osteoporosis

DOI: 10.3892/mmr.2020.11430
PMID: 32945471	Sample: Serum

NEUROSCIENCE LETTERS (2021) IF: 3.046

Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

DOI: 10.1016/j.neulet.2021.136385
PMID: 34871743	Sample: Serum

RARE METALS (2021) IF: 4.003

Degradation and biological performance of porous osteomimetic biphasic calcium phosphate in vitro and in vivo

DOI: 10.1007/s12598-021-01814-0
	Sample: Mg63 Cell

BONE (2021) IF: 4.398

Coupling Induction of Osteogenesis and Type H Vessels by Pulsed Electromagnetic Fields in Ovariectomy-induced Osteoporosis in Mice

DOI: 10.1016/j.bone.2021.116211
PMID: 34560308	Sample: Serum

PLoS Neglected Tropical Diseases (2023) IF: 4.781

Improvement of Antialveolar echinococcosis efficacy of novel Albendazole-Bile acids Derivatives with Enhanced Oral Bioavailability

DOI: 10.1371/journal.pntd.0011031

Scientific Reports (2022) IF: 4.996

Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

DOI: 10.1038/s41598-022-21816-x
	Sample: Serum

Frontiers in Endocrinology (2021) IF: 5.555

Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism

DOI: 10.3389/fendo.2021.759843
PMID: 34777254	Sample: Serum

Frontiers in Pharmacology (2021) IF: 5.811

Phillygenin Attenuates Carbon Tetrachloride-Induced Liver Fibrosis via Modulating Inflammation and Gut Microbiota

DOI: 10.3389/fphar.2021.756924
PMID: 34621179	Sample: Serum

JOURNAL OF CELLULAR PHYSIOLOGY (2022) IF: 6.513

IGF2BP2, an RNA-binding protein regulates cell proliferation and osteogenic differentiation by stabilizing SRF mRNA

DOI: 10.1002/jcp.30919

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022) IF: 9.028

Lipid Metabolic Disorder Induced by Pyrethroids in Nonalcoholic Fatty Liver Disease of Xenopus laevis

DOI: 10.1021/acs.est.2c00516
	Sample: Plasma

JOURNAL OF HAZARDOUS MATERIALS (2020) IF: 9.038

The combined adverse effects of cis-bifenthrin and graphene oxide on lipid homeostasis in Xenopus laevis

DOI: 10.1016/j.jhazmat.2020.124876
PMID: 33360192

JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429

BMSC exosome-enriched acellular fish scale scaffolds promote bone regeneration

DOI: 10.1186/s12951-022-01646-9
PMID: 36224596	Sample: Bmscs

Science Advances (2019) IF: 14.143

A prokaryotic-eukaryotic hybrid viral vector for delivery of large cargos of genes and proteins into human cells

DOI: 10.1126/sciadv.aax0064
	Sample: Serum

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Reviews/Q&A

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Reviews
Q&A

L*****aSubmitted [ Jul 08 2024 ]

Asked: Could you please inform price include shipping to Indonesia for item Alkaline phosphatase assay (ALP) E-BC-K091-M?

adminSubmitted [ Jul 08 2024 ]

Answered: I will send you mail,thank you .

M*****iSubmitted [ Oct 09 2022 ]

Asked: How to convert King Unit /100 mL to U/L?

adminSubmitted [ Oct 09 2022 ]

Answered: King Unit /100 mL = 7.14 U/L

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