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APC Anti-Human CD8a Antibody[OKT-8]

Uniprot : P01732
  • Cat.No.:E-AB-F1110E

  • Applications: FCM
  • Isotype: Mouse IgG2a, κ
  • Host: Mouse

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Antigen Details

Alternate Names T-cell surface glycoprotein CD8 alpha chain,CD8A,T-lymphocyte differentiation antigen T8/Leu-2,MAL
Swissprot P01732
References
1. Knapp W. W. Knapp .. et al.Oxford University Press; 1989; :1-1182.
2. Reimann KA, Waite BC, Lee-Parritz DE, et al. Cytometry. 1994; 17(1):102-108.
3. Roederer M, Kantor AB, Parks DR, Herzenberg LA. Cytometry. 1996; 24(3):191-197.
4. Schlossman SF. Stuart F. Schlossman .. et al. Oxford University Press; 1995.
5. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Cytometry. 1997; 29(4):351-362.

Product Details

Form Liquid
Clone No. OKT-8(See Other Available Formats)
Host Mouse
Isotype Mouse IgG2a, κ
Isotype Control APC Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802E]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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