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ATP Chemiluminescence Assay Kit

Citations(2)

Cat.No.:E-BC-F002
Detection instrument: Chemiluminescence detector

Detection method: Fluorescence method

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48T

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Detection principle

Under the catalyzation of luciferase, ATP react with luciferin and emits fluorescence, and the fluorescence intensity is proportional to the concentration of ATP within a certain range.

Performance characteristics

Synonyms	ATP
Sample type	Animal tissue, Cells
Sensitivity	0.003 μmol/L
Detection range	0.003-5 μmol/L
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	15 min
Precision	Average inter-assay CV: 6.500%Average intra-assay CV: 2.200%
Other instruments required	Vortex mixer, Centrifuge
Storage	-20℃
Valid period	12 months

Images

ATP level was significantly reduced in SB225002 cells. (*P<0.05, **P<0.01)

H Wang et al investigate the overexpression of CXCR2 on monocytes of traumatic brain injury and its effect. ATP level in cell supernatant was determined using ATP chemiluminescence assay kit (E-BC-F002).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.003-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Mouse heart tissue homogenate	1
10% Mouse kidney tissue homogenate	1
10% Mouse muscle tissue homogenate	1
10% Mouse liver tissue homogenate	1
10% Mouse brain tissue homogenate	1
10% Mouse lung tissue homogenate	1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Adipocyte (2022) IF: 3.553

Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway

DOI: 10.1080/21623945.2022.2116790
PMID: 36053001	Sample: 3T3-L1 Adipocytes

Journal of Neuroinflammation (2022) IF: 9.587

Transient post-operative overexpression of CXCR2 on monocytes of traumatic brain injury patients drives monocyte chemotaxis toward cerebrospinal fluid and enhances monocyte-mediated immunogenic cell death of neurons in vitro

DOI: 10.1186/s12974-022-02535-6
PMID: 35768823	Sample: Sh-Sy5Y Cell

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