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Cat.No.:E-BC-K318-M
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Protocols for metabolism assays
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★: Microplate is not provided in metabolism assay kits of over 500 assays.
Synonyms | TP |
Sample type | Serum,plasma,cell culture supernatant,tissue,cells |
Sensitivity | 0.0165 mg/mL |
Detection range | 0.0165-1 mg/mL |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 40 min |
Precision | Average inter-assay CV: 4.500%Average intra-assay CV: 2.200% |
Other instruments required | Vortex mixer, Micropipettor, Incubator |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | RT |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.0165-1 mg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
10% Mouse brain tissue homogenization |
8-12 |
10% Mouse kidney tissue homogenization |
8-12 |
Human serum |
100-200 |
10% Rat liver tissue homogenization |
15-20 |
10% Mouse heart tissue homogenization |
8-12 |
Rat serum |
100-200 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Sample: Kidney Tissue |
Sample: Lung Tissue,Thp-1 Cells |
Sample: Tissue Homogenate |
PMID: 33994520 |
Sample: Periventricular |
Sample: Serum,Plasma,Whole Blood,Platelet,Tissue Homogenat |
PMID: 35366690 |
Sample: Ectopic Endometria |
Sample: Cd4+ T Cell |
Sample: Brain Tissues,Plasma,Muscle Tissues |
Sample: Primary Huvecs |
DOI: 10.1111/and.14216 |
|
PMID: 34396564 |
Sample: Testis Tissue |
DOI: 10.2147/JPR.S400871 |
|
Sample: Dorsal Root Ganglion (Drg) Tissue,Intervertebral |
PMID: 35438362 |
Sample: Sw480 Cell,Hct116 Cell |
PMID: 33181259 |
Sample: Serum |
Sample: Bone Marrow |
PMID: 32373979 |
Sample: Tissue Homogenate |
PMID: 35153135 |
Sample: Ovarian Tissue |
Sample: Hippocampal Tissue,Primary Neurons |
PMID: 30928632 |
Sample: Cell Culture Supernatant,Tissue Homogenate |
PMID: 31824338 |
Sample: Plasma |
PMID: 35421180 |
Sample: Brain Tissue |
PMID: 33050458 |
Sample: Cell Lysate |
PMID: 35191812 |
PMID: 34903141 |
Sample: Arpe19 Cell |
PMID: 35193668 |
PMID: 34507635 |
Sample: El4 Cell |
Sample: Tissue Homogenate |
DOI: 10.1002/brb3.2778 |
|
PMID: 36271663 |
DOI: 10.1002/jbm.b.35232 |
|
Sample: Hpdlscs |
Sample: Mammary Gland Tissue |
DOI: 10.1155/2022/5332129 |
|
PMID: 35664988 |
Sample: Mkn-45 Cell |
Sample: Cell Lysate(Brl Cell Lines (Normal Rat Hepatocytes |
DOI: 10.1111/jfbc.14315 |
|
PMID: 35855584 |
Sample: Hacat Cell |
Sample: Tissue Homogenate |
PMID: 36152772 |
Sample: Brain Tissue |
Sample: Htr-8/Svneo Cell |
Sample: Bone Marrow-Derived Macrophages (Bmms) |
PMID: 35690133 |
Sample: Fibroblast |
DOI: 10.21037/atm-22-222 |
|
PMID: 35280405 |
Sample: Cardioid |
DOI: 10.21037/atm-22-483 |
|
PMID: 35280364 |
Sample: Myocardium |
DOI: 10.3390/biom10060816 |
|
PMID: 32466476 |
Sample: Tissue Homogenate |
DOI: 10.1002/tox.23297 |
|
Sample: Cell Culture Supernatant |
Sample: Primary Endometrial Cell |
Sample: A549 Cell,Pc-9 Cell,Nci-H292 Cell,Nci-1975 Cell,Be |
Sample: Granulosa Cells (Gcs) |
PMID: 33430586 |
Sample: Tissue Homogenate(Brain) |
DOI: 10.1111/jre.12973 |
|
PMID: 35037251 |
Sample: Periodontal Ligament Stem Cell (Pdlscs) |
PMID: 34858216 |
Sample: Heart |
PMID: 35392411 |
Sample: Cortex,Hippocampus |
Sample: Tissue Homogenate(Corneas) |
PMID: 34464887 |
Sample: Balf |
PMID: 34794890 |
PMID: 35101849 |
Sample: Spinal Cord,Bv2 Microglial Cell |
PMID: 33582592 |
Sample: Cell Culture Supernatant |
Sample: Fruit |
PMID: 35858881 |
Sample: Fibroblast |
PMID: 35717715 |
Sample: Cardiac Tissue |
Sample: Corneas,Raw 264.7 Cell |
PMID: 35464198 |
Sample: Gmscs |
DOI: 10.1111/jcmm.17183 |
|
Sample: Brain Tissue |
Sample: Serum,Liver Tissue,Fecal Lipids |
DOI: 10.1039/D1FO03527D |
|
PMID: 35260876 |
Sample: Hepg2 Cell |
Sample: 451Lu Cells,Uacc62 Cells |
DOI: 10.1159/000495718 |
|
Sample: Colonic Tissues,Ht-29 Cells |
PMID: 35810488 |
Sample: Corneas |
Sample: Corneal Tissue,Human Corneal Epithelial Cells (Hce |
PMID: 35471485 |
Sample: Admsc-Exos |
Sample: Seed Coat |
DOI: 10.3390/ph15010016 |
|
Sample: Serum |
Sample: Blca Cell,Ccrcc Cell,Pca Cell |
PMID: 36142831 |
Sample: Muscle |
Sample: Embryo |
DOI: 10.1155/2021/9966663 |
|
PMID: 34422216 |
Sample: Brain |
Sample: Hl7702 Cell,Hepg2 Cell,Huh-7 Cell |
PMID: 34403738 |
Sample: Medial Prefrontal Cortex (Mpfc) |
Sample: Rm-1 Cell,Lncap Pca Cell |
PMID: 35108485 |
Sample: Liposome |
PMID: 36224596 |
Sample: Bmscs |
PMID: 36792573 |
Sample: Bm-Derived Macrophages (Bmdms) |
T****************nSubmitted [ Jan 10 2024 ]
Asked: can this kit use to measure protein in zebrafish tissue samples,tq
Reply
adminSubmitted [ Jan 10 2024 ]
Answered: This kit can be used to measure tissue samples regardless of species. Our laboratory has not verified the zebrafish tissue samples, but theoretically, if the protein content of the samples is higher than the sensitivity of the kit, it can be detected.
aSubmitted [ Dec 08 2023 ]
Asked: can this be used to assess stool protein level
Reply
M***********dSubmitted [ Jun 11 2023 ]
Asked: Can this kit be used for bronchoalveolar lavage samples? And what should be the dilution factor of mouse lung tissue samples
Reply
adminSubmitted [ Jun 11 2023 ]
Answered: Hello Muntaha javid,thanks for your support.Our kit is suitable for bronchoalveolar lavage samples.Lung tissue sample processing: 10% sample homogenate diluted 10 times.
M*****aSubmitted [ Aug 01 2022 ]
Asked: E-BC-K318-M is ok for use with Human salive samples. If so, can you please send me salive samples method ?
Reply
adminSubmitted [ Aug 01 2022 ]
Answered: Saliva: gargling with clear water, after 30 min saliva was collected and centrifuged at 10000 g at 4℃ for 5 min. Then take the supernatant for detection. If not detected on the same day, the saliva can be stored at-80℃ for a month.
M*****aSubmitted [ Aug 01 2022 ]
Asked: How long is the shelf life of this kit
Reply
adminSubmitted [ Aug 01 2022 ]
Answered: The shelf life of this kit is 12 months.