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Catalase (CAT) Activity Assay Kit

Citations(89)

  • Cat.No.:E-BC-K031-M

  • Detection instrument: Microplate reader(400-410 nm,optimum wavelength: 405 nm)

Manual MSDS

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  • 96T
  • 48T
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Detection principle

The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

Performance characteristics

Synonyms CAT
Sample type Serum,plasma,cells,cell culture supernatant,tissue
Sensitivity 1.12 U/mL
Detection range 1.12 -150 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 40 min
Precision Average inter-assay CV: 7.700%Average intra-assay CV: 3.900%
Other instruments required Micropipettor, Vortex Mixer, Incubator
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

The activity of CAT increased significantly after treated with melatonin in 5 μM of cadmium. (*P<0.05)

D Bartolini et al investigate the mechanism of melatonin protecting porcine pre-pubertal Sertoli cell from cadmium through regulating Nrf2 acivity. Catalase (CAT) activity of porcine pre-pubertal Sertoli cell was determined using CAT activity assay kit (E-BC-K031-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.12 -150 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

293T supernatant

1

10% Rat heart tissue homogenization

50-100

10% Rat liver tissue homogenization

100-200

10% Rat spleen tissue homogenization

50-100

Mouse serum

1

10% Epipremnum aureum tissue homogenization

1-2

10% Rat lung tissue homogenization

50-100

10% Rat kidney tissue homogenization

50-100

10% Rat brain tissue homogenization

20-50

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Citations

  1. Frontiers in Pharmacology (2022) IF: 5.988
    Anti-arthritic and toxicological evaluation of ethanolic extract of Alternanthera bettzickiana in rats.

    DOI: 10.3389/fphar.2022.1002037
    Sample: Serum
  2. FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
    Roflumilast protects from cisplatin-induced testicular toxicity in male rats and enhances its cytotoxicity in prostate cancer cell line. Role of NF-κB-p65, cAMP/PKA and Nrf2/HO-1, NQO1 signaling

    DOI: 10.1016/j.fct.2021.112133

    PMID: 33757793

    		 Sample: Testicular
  3. Frontiers in Molecular Biosciences (2022) IF: 6.113
    Biochemical profiling of metabolomics in heavy metal-intoxicated impaired metabolism and its amelioration using plant-based bioactive compound

    DOI: 10.3389/fmolb.2022.1029729

    PMID: 36330218

    		 Sample: Liver
  4. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Antidiabetic effects of Psidium x durbanensis Baijnath & Ramcharun ined. (Myrtaceae) leaf extract on streptozotocin-induced diabetes in rats

    DOI: 10.1016/j.jep.2022.115542

    PMID: 35842177

    		 Sample: Liver
  5. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025
    Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

    DOI: 10.1016/j.fct.2022.113061

    PMID: 35489465

    		 Sample: Pig Testicular Cell (St Cell)
  6. Journal of Toxicology (2020)
    Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

    DOI: 10.1155/2020/1807126
    Sample: C2C12 Cell
  7. African Journal of Pharmacy and Pharmacology (2019)
    Hepatoprotective and antioxidant activities of Pterocarpus santalinoides methanol leaf extract

    DOI: 10.5897/AJPP2019.5090
    Sample: Serum
  8. Toxicology Reports (2019)
    Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

    DOI: 10.1016/j.toxrep.2019.06.009
    Sample: Plasma
  9. Toxicology Reports (2022)
    Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats

    DOI: 10.1016/j.toxrep.2022.06.005
    Sample: Plasma
  10. Toxicology Reports (2017)
    Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

    DOI: 10.1016/j.toxrep.2017.12.002
    Sample: Plasma
  11. SAGE Open Medicine (2021)
    Deficiency of antioxidants and increased oxidative stress in COVID-19 patients: A cross-sectional comparative study in Jigawa, Northwestern Nigeria:

    DOI: 10.1177/2050312121991246
    Sample: Serum
  12. Advances in Traditional Medicine (2020)
    Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic pain

    DOI: 10.1007/s13596-019-00411-y
    Sample: Serum,Sciatic Nerves
  13. Brazilian Journal of Pharmaceutical Sciences (2022) IF: 1.214
    Stingless bee propolis, metformin, and their combination alleviate diabetic cardiomyopathy

    DOI: 10.1590/s2175-97902022e19652
    Sample: Heart Tissue
  14. HORTSCIENCE (2021) IF: 1.455
    Effects of Salt Stress on Photosynthetic Fluorescence Characteristics, Antioxidant System, and Osmoregulation of Coreopsis tinctoria Nutt.

    DOI: 10.21273/HORTSCI15956-21
    Sample: Seed
  15. WORK-A Journal of Prevention Assessment & Rehabilitation (2021) IF: 1.505
    Evaluation of oxidative stress and pro-inflammatory cytokines in occupationally cadmium exposed workers

    DOI: 10.3233/WOR-203302

    PMID: 33104053

    		 Sample: Serum
  16. JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
    The effects of chrysin on lipopolysaccharide‐induced sepsis in rats

    DOI: 10.1111/jfbc.13359
    Sample: Lung,Liver,Kidney
  17. International Journal of General Medicine (2023) IF: 2.145
    Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

    DOI: 10.2147/IJGM.S402206
    Sample: Serum
  18. CRYOBIOLOGY (2020) IF: 2.287
    Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen

    DOI: 10.1016/j.cryobiol.2020.04.010

    PMID: 32437678

    		 Sample: Sperm
  19. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL (2020) IF: 2.363
    Chrysin Suppresses HT-29 Cell Death Induced by Diclofenac through Apoptosis and Oxidative Damage

    DOI: 10.1080/01635581.2020.1801775
    Sample: Ht-29 Cell
  20. MICROBIAL PATHOGENESIS (2019) IF: 2.581
    Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

    DOI: 10.1016/j.micpath.2019.103557
    Sample: Small Intestinal Tissues
  21. Dose-Response (2022) IF: 2.623
    Pharmacological Role of Capparis decidua (Forssk.) Edgew in Preventing Cyclophosphamide-induced Myelosuppression and Modulating Innate and Adaptive Immune Response:

    DOI: 10.1177/15593258221123672
    Sample: Serum
  22. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Etlingera elatior Flower Aqueous Extract Protects against Oxidative Stress-Induced Nephropathy in a Rat Model of Type 2 Diabetes

    DOI: 10.1155/2022/2814196

    PMID: 35502173

    		 Sample: Plasma
  23. Applied Sciences-Basel (2021) IF: 2.679
    Biochemical Mapping of the Inflamed Human Dental Pulp

    DOI: 10.3390/app112110395
    Sample: Dental Pulp Lysates
  24. Toxicology Research (2022) IF: 2.68
    Glutathione attenuated lambda-cyhalothrin-induced alteration of serum total cholesterol concentration and oxidative stress parameters in rats

    DOI: 10.1093/toxres/tfac080
    Sample: Serum
  25. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
    Watermelon (Citrullus lanatus) leaf extract attenuates biochemical and histological parameters in high-fat diet/streptozotocin-induced diabetic rats

    DOI: 10.1111/jfbc.14058

    PMID: 34981526

    		 Sample: Serum
  26. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    A standardized black pepper seed extract containing β-caryophyllene improves cognitive function in scopolamine-induced amnesia model mice via regulation of brain-derived neurotrophic factor and MAPK proteins

    DOI: 10.1111/jfbc.13994

    PMID: 34778972

    		 Sample: Brain
  27. Animals (2021) IF: 2.752
    The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens

    DOI: 10.3390/ani11020399

    PMID: 33557433

    		 Sample: Plasma
  28. Analytical Methods (2022) IF: 2.896
    Evaluate the Effect of nitrate and chloride on Cd(II)-Induced Single Cell Oxidative Stress by Scanning Electrochemical Microscopy

    DOI: 10.1039/D2AY00495J
    Sample: Mcf-7 Cell
  29. HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
    Scutellarin alleviates type 2 diabetes (HFD/low dose STZ)-induced cardiac injury through modulation of oxidative stress, inflammation, apoptosis and fibrosis in mice:

    DOI: 10.1177/09603271211045948
    Sample: Heart
  30. THERIOGENOLOGY (2023) IF: 2.923
    Seasonal variations in the metabolomic profile of the ovarian follicle components in Italian Mediterranean Buffaloes

    DOI: 10.1016/j.theriogenology.2023.02.022
    Sample: Follicular Fluid
  31. CURRENT ISSUES IN MOLECULAR BIOLOGY (2023) IF: 2.976
    Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

    DOI: 10.3390/cimb45020057
    Sample: Colonic Tissue
  32. PeerJ (2021) IF: 2.984
    Physiological changes and transcript identification in Coreopsis tinctoria Nutt. in early stages of salt stress

    DOI: 10.7717/peerj.11888

    PMID: 34434650

    		 Sample: Seedling
  33. KIDNEY & BLOOD PRESSURE RESEARCH (2023) IF: 3.096
    Protective effects of Xanthohumol against diabetic nephropathy in a mouse model

    DOI: 10.1159/000528650
    Sample: Kidney
  34. EUROPEAN JOURNAL OF NEUROSCIENCE (2020) IF: 3.115
    Neuroprotective effects of brain‐derived neurotrophic factor against amyloid beta 1‐40‐induced retinal and optic nerve damage

    DOI: 10.1111/ejn.14662
    Sample: Tissue Homogenate
  35. REPRODUCTIVE TOXICOLOGY (2020) IF: 3.121
    Tadalafil alleviates cisplatin-induced reproductive toxicity through the activation of the Nrf2/HO-1 pathway and the inhibition of oxidative stress and apoptosis in male rats

    DOI: 10.1016/j.reprotox.2020.06.015
    Sample: Testicular
  36. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2022) IF: 3.168
    ViphyllinTM, a Standardized Black Pepper Extract Exerts Antihyperglycemic Effect and Improves Sciatic Nerve Conduction in High Fat Diet/Streptozotocin-Induced Diabetic Model Rats

    DOI: 10.2147/DMSO.S366609
    Sample: Sciatic Nerve Tissue
  37. Life-Basel (2022) IF: 3.251
    Mechanisms and Effects of Isorhamnetin on Imiquimod-Induced Psoriasiform Dermatitis in Mice

    DOI: 10.3390/life12122107
    Sample: Skin Tissue
  38. NEUROTOXICITY RESEARCH (2019) IF: 3.311
    The Elastin-Derived Peptide VGVAPG Does Not Activate the Inflammatory Process in Mouse Cortical Astrocytes In Vitro

    DOI: 10.1007/s12640-019-00114-x
    Sample: Mouse Astrocyte Cell
  39. ANIMAL FEED SCIENCE AND TECHNOLOGY (2022) IF: 3.313
    Housefly maggot meal complement soybean meal in a fish-free diet for hybrid catfish (Clarias gariepinus ♀ x Heterobranchus longifilis ♂): Effect on growth, body composition, blood biochemistry and antioxidant enzyme activity

    DOI: 10.1016/j.anifeedsci.2022.115543
    Sample: Serum
  40. Processes (2022) IF: 3.352
    Gastroprotective, Biochemical, and Acute Toxicity Effects of Papaver decaisnei against Ethanol-Induced Gastric Ulcers in Rats

    DOI: 10.3390/pr10101985
    Sample: Gastric Tissue
  41. Processes (2022) IF: 3.352
    Gastroprophylactic Effects of p-Cymene in Ethanol-Induced Gastric Ulcer in Rats

    DOI: 10.3390/pr10071314
    Sample: Stomach
  42. International Journal of Environmental Research and Public Health (2021) IF: 3.39
    Polystyrene Microplastics Exposure: An Insight into Multiple Organ Histological Alterations, Oxidative Stress and Neurotoxicity in Javanese Medaka Fish (Oryzias javanicus Bleeker, 1854)

    DOI: 10.3390/ijerph18189449
    Sample: Intestine,Brain
  43. PHARMACEUTICAL BIOLOGY (2021) IF: 3.503
    The anti-breast cancer property of physcion via oxidative stress-mediated mitochondrial apoptosis and immune response

    DOI: 10.1080/13880209.2021.1889002

    PMID: 33715588

    		 Sample: Serum
  44. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    		 Sample: Brain Tissue
  45. ACS Omega (2022) IF: 3.512
    Sarcococca saligna Hydroalcoholic Extract Ameliorates Arthritis in Complete Freund’s Adjuvant-Induced Arthritic Rats via Modulation of Inflammatory Biomarkers and Suppression of Oxidative Stress Markers

    DOI: 10.1021/acsomega.2c00619
    Sample: Serum
  46. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

    DOI: 10.1007/s11011-022-00952-3

    PMID: 35298730

    		 Sample: Hippocampal,Hepatic
  47. JOURNAL OF APPLIED TOXICOLOGY (2022) IF: 3.628
    Asiaticoside attenuates oxygen-glucose deprivation/reoxygenation-caused injury of cardiomyocytes by inhibiting autophagy

    DOI: 10.1002/jat.4424
    Sample: Ac16 Cells
  48. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 3.639
    Flaxseed oil ameliorates aging in D-galactose induced rats via altering gut microbiota and mitigating oxidative damage

    DOI: 10.1002/jsfa.12010

    PMID: 35567370

    		 Sample: Liver
  49. LIFE SCIENCES (2020) IF: 3.647
    Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

    DOI: 10.1016/j.lfs.2020.117584

    PMID: 32220623

    		 Sample: Tissue Homogenate
  50. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Viphyllin™, a standardized extract from black pepper seeds, mitigates intestinal inflammation, oxidative stress, and anxiety-like behavior in DSS-induced colitis mice

    DOI: 10.1111/jfbc.14306

    PMID: 35766031

    		 Sample: Colon Tissue,Brain Tissue
  51. PLANT PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 3.72
    Overexpression of ethylene response factor ERF96 gene enhances selenium tolerance in Arabidopsis

    DOI: 10.1016/j.plaphy.2020.02.024

    PMID: 32097848

    		 Sample: Plant Tissue
  52. NEUROCHEMISTRY INTERNATIONAL (2021) IF: 3.921
    Exposure to footshock stress downregulates antioxidant genes and increases neuronal apoptosis in an Aβ(1–42) rat model of Alzheimer's disease

    DOI: 10.1016/j.neuint.2021.105170
    Sample: Amygdala,Hippocampus
  53. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway

    DOI: 10.1016/j.intimp.2020.107290

    PMID: 33383446

    		 Sample: Lung Tissue
  54. CELLULAR SIGNALLING (2020) IF: 3.968
    Circular RNA circ_DROSHA alleviates the neural damage in a cell model of temporal lobe epilepsy through regulating miR-106b-5p/MEF2C axis

    DOI: 10.1016/j.cellsig.2020.109901

    PMID: 33370579

    		 Sample: Sk-N-Sh Cell,Imr-32 Cell
  55. BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 4.081
    Selenium Supplementation Protects Against Arsenic-Trioxide-Induced Cardiotoxicity Via Reducing Oxidative Stress and Inflammation Through Increasing NAD+ Pool

    DOI: 10.1007/s12011-022-03478-y
    Sample: Heart Tissue
  56. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Differential neuroprotective effect of curcuminoid formulations in aluminum chloride–induced Alzheimer’s disease

    DOI: 10.1007/s11356-022-20593-4
    Sample: Brain Tissue
  57. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Centella asiatica extract protects against cisplatin-induced hepatotoxicity via targeting oxidative stress, inflammation, and apoptosis

    DOI: 10.1007/s11356-022-18626-z

    PMID: 35029831

    		 Sample: Hepatic Tissue
  58. NEUROCHEMISTRY INTERNATIONAL (2022) IF: 4.297
    Calcium channel antagonists interfere with the mechanism of action of elastin-derived peptide VGVAPG in mouse cortical astrocytes in vitro

    DOI: 10.1016/j.neuint.2022.105405

    PMID: 35934159

    		 Sample: Astrocyte Cell
  59. Drug Design Development and Therapy (2022) IF: 4.319
    The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

    DOI: 10.2147/DDDT.S344247

    PMID: 35965961

    		 Sample: Serum
  60. Foods (2021) IF: 4.35
    Quality of Dabai Pulp Oil Extracted by Supercritical Carbon Dioxide and Supplementation in Hypercholesterolemic Rat—A New Alternative Fat

    DOI: 10.3390/foods10020262
    Sample: Serum
  61. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    		 Sample: Serum
  62. Scientific Reports (2022) IF: 4.38
    Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants

    DOI: 10.1038/s41598-022-14570-7

    PMID: 35729338

    		 Sample: Leaf
  63. Nutrients (2020) IF: 4.546
    Effect of Defatted Dabai Pulp Extract in Urine Metabolomics of Hypercholesterolemic Rats

    DOI: 10.3390/nu12113511

    PMID: 33202660

    		 Sample: Serum
  64. Aging-US (2020) IF: 4.831
    Electrical stimulation inhibits Val-boroPro-induced pyroptosis in THP-1 macrophages via sirtuin3 activation to promote autophagy and inhibit ROS generation

    DOI: 10.18632/aging.103038
    Sample: Thp-1 Cell
  65. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Vernodalin regulated the NF-κβp65 signaling in inflammation of lipopolysaccharide -induced sepsis rats

    DOI: 10.1016/j.procbio.2022.10.004
    Sample: Liver,Kidney,Lungs
  66. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Optimization of ultrasound-assisted extraction, structural, functional, and antioxidant properties of Glycyrrhiza uralensis seed protein

    DOI: 10.1016/j.procbio.2022.11.001
    Sample: Serum,Liver
  67. MOLECULES (2022) IF: 4.927
    Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in Rats

    DOI: 10.3390/molecules27196470

    PMID: 36235007

    		 Sample: Plasma
  68. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    Gastroprotective effect of an active ingredients group of Lindera reflexa Hemsl. On Ethanol-Induced gastric ulcers in Rats: Involvement of VEGFR2/ERK and TLR-2/Myd88 signaling pathway

    DOI: 10.1016/j.intimp.2022.108673

    PMID: 35259712

    		 Sample: Stomach Tissues
  69. Marine Drugs (2021) IF: 5.118
    Heptapeptide Isolated from Isochrysis zhanjiangensis Exhibited Anti-Photoaging Potential via MAPK/AP-1/MMP Pathway and Anti-Apoptosis in UVB-Irradiated HaCaT Cells

    DOI: 10.3390/md19110626

    PMID: 34822497

    		 Sample: Hacat Cells
  70. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: Livers
  71. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    		 Sample: Serum
  72. Journal of Environmental Sciences (2022) IF: 5.565
    Alleviative effects of a novel strain Bacillus coagulans XY2 on copper-induced toxicity in zebrafish larvae

    DOI: 10.1016/j.jes.2022.02.010

    PMID: 36375957

    		 Sample: Larvae
  73. LIVER TRANSPLANTATION (2022) IF: 5.799
    Cerium oxide nanoparticles administration during machine perfusion of discarded human livers: a pilot study

    DOI: 10.1002/lt.26421

    PMID: 35100468

    		 Sample: Liver
  74. Frontiers in Pharmacology (2022) IF: 5.988
    Mailuoshutong pill for varicocele-associated male infertility-Phytochemical characterisation and multitarget mechanism.

    DOI: 10.3389/fphar.2022.961011
    Sample: Testicular Tissue
  75. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 6.291
    Antibacterial effects of nano-decoction iron polysulfide in epididymitis and the systematic evaluation of its toxicity on the reproductive health of male mice

    DOI: 10.1016/j.ecoenv.2022.113184

    PMID: 35032726

    		 Sample: Testis
  76. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Lipoic acid (LA) dose-dependently protects bone losses in the mandible of rats during the development of osteopenia by inhibiting oxidative stress and promoting bone formation

    DOI: 10.1016/j.biopha.2021.112467

    PMID: 34891114

    		 Sample: Serum
  77. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 6.53
    Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role

    DOI: 10.1016/j.biopha.2020.110075

    PMID: 32179202

    		 Sample: Tissue Homogenate
  78. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed rats

    DOI: 10.1016/j.biopha.2021.112017

    PMID: 34399203

    		 Sample: Heart
  79. Cells (2021) IF: 6.6
    Loganin Ameliorates Painful Diabetic Neuropathy by Modulating Oxidative Stress, Inflammation and Insulin Sensitivity in Streptozotocin-Nicotinamide-Induced Diabetic Rats

    DOI: 10.3390/cells10102688

    PMID: 34685668

    		 Sample: Plasma
  80. PLANT CELL AND ENVIRONMENT (2022) IF: 7.228
    The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

    DOI: 10.1111/pce.14263

    PMID: 35040151

    		 Sample: Leaf
  81. Antioxidants (2022) IF: 7.675
    Selenoprotein F Knockout Caused Glucose Metabolism Disorder in Young Mice by Disrupting Redox Homeostasis

    DOI: 10.3390/antiox11112105

    PMID: 36358477

    		 Sample: Liver,Serum
  82. Antioxidants (2022) IF: 7.675
    Role of Oxidative Stress and Lipid Peroxidation in the Pathophysiology of NAFLD

    DOI: 10.3390/antiox11112217
    Sample: Plasma
  83. Antioxidants (2022) IF: 7.675
    Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats

    DOI: 10.3390/antiox11101879

    PMID: 36290602

    		 Sample: Ovarian Tissue
  84. Antioxidants (2022) IF: 7.675
    Interleukin-33 Induces Neutrophil Extracellular Trap (NET) Formation and Macrophage Necroptosis via Enhancing Oxidative Stress and Secretion of Proatherogenic Factors in Advanced Atherosclerosis

    DOI: 10.3390/antiox11122343
    Sample: Peripheral Blood Mononuclear Cells (Pbmcs)
  85. Antioxidants (2022) IF: 7.675
    Effects of Gold Nanoparticles Functionalized with Bioactive Compounds from Cornus mas Fruit on Aorta Ultrastructural and Biochemical Changes in Rats on a Hyperlipid Diet—A Preliminary Study

    DOI: 10.3390/antiox11071343
    Sample: Serum,Aorta
  86. ACS Applied Materials & Interfaces (2021) IF: 9.229
    Osmium–Tellurium Nanozymes for Pentamodal Combinatorial Cancer Therapy

    DOI: 10.1021/acsami.1c14201
    Sample: Ostenrs
  87. JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588
    Sequential use of UV-LEDs irradiation and chlorine to disinfect waterborne fungal spores: Efficiency, mechanism and photoreactivation

    DOI: 10.1016/j.jhazmat.2021.127102

    PMID: 34482083
  88. JOURNAL OF PINEAL RESEARCH (2022) IF: 13.007
    Melatonin modulates Nrf2 activity to protect porcine pre-pubertal Sertoli cells from the abnormal H2O2 generation and reductive stress effects of cadmium.

    DOI: 10.1111/jpi.12806
    Sample: Sertoli Cell (Scs)
  89. ACS Nano (2020) IF: 14.588
    Nonrecurring Circuit Nanozymatic Enhancement of Hypoxic Pancreatic Cancer Phototherapy Using Speckled Ru–Te Hollow Nanorods

    DOI: 10.1021/acsnano.9b09974
    Sample: Rutenrs
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Reviews/Q&A

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  • Reviews
  • Q&A

Verified Customer

m***ySubmitted [ May 31 2019 ]

  • Application:Biochemical
  • Description:I used this Catalase (CAT) kit to test rat liver homogenate. the detection process require a small sample size and takes a little time .
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Q

M*tSubmitted [ Oct 03 2024 ]

Asked: I want to determine catalase activity in cultured cells, can I freeze the supernatants at -80°C after sonication for further processing or should I do the determination on the same day as the lysis?

Reply

A

adminSubmitted [ Oct 03 2024 ]

Answered: Hi Mat, It is recommended to do the determination on the same day as the lysis. If the supernatant after sonication is frozen at -80℃, the enzyme activity may be affected. If the test is not performed on the same day, it is recommended to freeze the cell precipitation directly (-80℃ for no more than one month), rather than the supernatant after lysis. Thank you.

Q

s* Submitted [ Aug 08 2024 ]

Asked: Is it necessary to determine protein concentration level? I do not have resource to perform it within short period

Reply

A

adminSubmitted [ Aug 08 2024 ]

Answered: E-BC-K031-M kit, if you detect serum or plasma sample, you can not detect the protein concentration. If you detect tissue or cell samples, due to the different homogenization degree of different samples, you need to detect the protein concentration in the supernatant after homogenization to correct the result. You can first test E-BC-K031-M, and then detect the protein concentration of the sample homogenate supernatant, do not require the detection time of protein concentration, the same day can be detected.

Q

N************RSubmitted [ Aug 05 2023 ]

Asked: Is this kit appropriate for studying with plants (lichens)?

Reply

A

adminSubmitted [ Aug 05 2023 ]

Answered: We'll reply to you by email. Pls pay attention to the mailbox

Q

r*** Submitted [ Jun 26 2023 ]

Asked: cual es el numero minimo de celulas para realizar el kit

Reply

A

adminSubmitted [ Jun 26 2023 ]

Answered: Hi Rosa, thanks for your support. The minimum number of cells to make a kit is 2 million, it is recommended to use 3 million cells for better results. Hola Rosa, gracias por tu apoyo. El número mínimo de celdas para hacer un kit es de 2 millones, se recomienda utilizar 3 millones de celdas para obtener mejores resultados.

Q

a**aSubmitted [ Jun 10 2023 ]

Asked: can i use this kit for plant samples

Reply

A

adminSubmitted [ Jun 10 2023 ]

Answered: Dear Anna,thanks for your support.Our kit is suitable for plant samples.

Q

M*****aSubmitted [ Aug 09 2022 ]

Asked: CAT kit, there is a problem in making the standard curve, can we use the standard curve in the manual?

Reply

A

adminSubmitted [ Aug 09 2022 ]

Answered: 

A

adminSubmitted [ Aug 09 2022 ]

Answered: The standard curve will vary according to the different experimental operations and different experimental environments. So, we suggest the customer making the standard curve by yourself.

Q

M*****aSubmitted [ Aug 02 2022 ]

Asked: What does the control mean? Is it means the control group in my own experimental design?

Reply

A

adminSubmitted [ Aug 02 2022 ]

Answered: This control refers to the control of each sample, not for the control group of experimental design. Every sample must set a control.

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