Introduction
Cell Staining Buffer, a
buffer optimized for sample preparation of flow cytometry (FCM), can be used in
fluorescent antibody dilution and cell sample washes.
This product contains
bovine serum albumin (BSA) as carrier protein to reduce non-specific binding of
fluorescent antibodies. Experiments showed that addition of BSA can also reduce
damage to cells during sample preparation and retain more cells for analysis.
This product contains EDTA
as a chelating reagent for metallic ions which promote cell to cell adhesion.
Addition of modest amount of EDTA can boost the ratio of singlets in FCM
analysis. This product contains preservative to prevent bacterial contamination
at a safe dosage to make sure that the impact on cells can be negligible.
Instructions
This product is provided as 1× working buffer. It can be added directly
into tubes containing cell pellets to resuspend.
Components
|
Cat.
|
Products
|
100 mL
|
200 mL
|
500 mL
|
Storage
|
|
E-CK-A107
|
Cell Staining Buffer
|
100 mL
|
200 mL
|
500 mL
|
2~8°C
|
|
Manual
|
One Copy
|
Experimental
Procedure
1. Single cell suspensions are centrifuged at 300 g for 5 min, discard the
supernatant.
2. Add desired amount of Cell Staining Buffer with a pipettor to resuspend
the cell pellets. For common analysis, 106 cells are resuspended
with 100 μL buffer, i.e. with a cell density of 107 cells/mL.
Storage
Store
at 2~8°C. Avoid of freezing. Valid for 12 months.
Cautions
1. This reagent is for research
use only.
2. For your safety and health,
please wear the lab coat and disposable gloves before the experiments.
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