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DAB Detection Kit

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      Catalog number:E-IR-R101

      Size:
      • 1mL
      • 3mL
      • 6mL
      Qty:
      - +
      Price: $35

      Lead Time:Order now, ship in 3 daysWelcome to order from local distributors.

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      Introduction

      Elabscience® DAB Detection kit can be used for immunohistochemical development. DAB (3,3N-Diaminobenzidine Tertrahydrochloride) is a common substrate of horseradish peroxidase (HRP), under the catalysis of horseradish peroxidase, DAB will produce a brown precipitate.


      Instructions

      DAB Concentrate (20×) is a concentrate solution, dilute the DAB Concentrate (20×) with DAB Substrate to 1× DAB working solution before use. For example: add 10 μL of DAB Concentrate (20×) to 190 μL of DAB Substrate and mix fully.

      Note: The prepared 1× DAB working solution should be used within 4 hours, and be protected from light.



      Components

        

      Cat.

      Products

      1 mL

      3 mL

      6 mL

      Storage

      E-IR-R101A

      DAB Concentrate (20×)

      1 mL

      3 mL

      6 mL

      2~8°C

      E-IR-R101B

      DAB Substrate

      20 mL

      60 mL

      120 mL

      2~8°C

      Manual

       One Copy

       

      Storage

      Store at 2~8°C in dark. Avoid of freezing. Valid for 12 months.

      The reagents are valid within 6 months after opening.


      Cautions

      1. For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.

      2. This kit is for research use only. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

      3. The positive and negative controls should be set up during the experiment to prevent possible false positive and false negative results.

      4. Please dry the liquid around the slice during the experiment to prevent weakening the dyeing intensity.


      Citations

      1. Progesterone modulates cadmium-induced oxidative stress and inflammation in hepatic tissues of Wistar rats
        Journal: International Journal of Clinical and Experimental Pathology(2021)
        PMID: 34760041
        Products: E-IR-R101 
      2. Dexmedetomidine prevents hemorrhagic brain injury by reducing damage induced by ferroptosis in mice
        IF: 3.197
        Journal: NEUROSCIENCE LETTERS(2022)
        DOI: 10.1016/j.neulet.2022.136842
        PMID: 35995304
        Products: E-IR-R101 
      3. Extracellular vesicles contribute to EMT in Adenomyosis by inducing macrophage polarization
        IF: 4.161
        Journal: BIOLOGY OF REPRODUCTION(2023)
        DOI: 10.1093/biolre/ioad015
        Products: E-IR-R101 
      4. SETD3 Methyltransferase Regulates PLK1 Expression to Promote In Situ Hepatic Carcinogenesis
        IF: 5.738
        Journal: Frontiers in Oncology(2022)
        DOI: 10.3389/fonc.2022.882202
        PMID: 35912180
        Products: E-IR-R101 
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